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<p>Highly sensitive and accurate detection of <i>Yersinia pestis</i> is critical for effective plague control and biothreat mitigation. Here, we developed a dual-mode colorimetric and fluorescence biosensor based on a multifunctional magnetic nanozyme for <i>Yersinia pestis</i> gene detection, integrating hybridization chain reaction (HCR) for signal amplification and alkaline phosphatase (ALP)-mediated signaling. ALP hydrolyzes adenosine triphosphate (ATP) and L-ascorbic acid-2-phosphate trisodium salt (AA2P) to generate turn-off colorimetric and turn-on fluorescence signals, respectively. The bioassay exhibited good linearity for target genes from 1&#xa0;pM to 10&#xa0;nM, with limits of detection of 0.34&#xa0;pM for colorimetric mode and 0.58&#xa0;pM for fluorescent mode. It also showed excellent specificity, distinguishing perfectly matched targets from single- and two-base mismatched sequences. Most importantly, the dual-mode platform was successfully applied to spiked human serum samples with satisfactory recoveries. This strategy integrates magnetic separation, signal amplification, and dual-signal output for highly sensitive and specific gene detection, offering great potential for plague monitoring and clinical diagnosis.</p> Graphical Abstract <p></p>

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Magnetic nanozyme-based dual-mode biosensor for highly sensitive detection of Yersinia pestis gene via hybridization chain reaction amplification

  • 期刊论文

  • 发表日期：

    2026年2月11日

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  • Yunqing Zhang,
  • Rui Yang,
  • Na Lu

摘要

Highly sensitive and accurate detection of Yersinia pestis is critical for effective plague control and biothreat mitigation. Here, we developed a dual-mode colorimetric and fluorescence biosensor based on a multifunctional magnetic nanozyme for Yersinia pestis gene detection, integrating hybridization chain reaction (HCR) for signal amplification and alkaline phosphatase (ALP)-mediated signaling. ALP hydrolyzes adenosine triphosphate (ATP) and L-ascorbic acid-2-phosphate trisodium salt (AA2P) to generate turn-off colorimetric and turn-on fluorescence signals, respectively. The bioassay exhibited good linearity for target genes from 1 pM to 10 nM, with limits of detection of 0.34 pM for colorimetric mode and 0.58 pM for fluorescent mode. It also showed excellent specificity, distinguishing perfectly matched targets from single- and two-base mismatched sequences. Most importantly, the dual-mode platform was successfully applied to spiked human serum samples with satisfactory recoveries. This strategy integrates magnetic separation, signal amplification, and dual-signal output for highly sensitive and specific gene detection, offering great potential for plague monitoring and clinical diagnosis.

Graphical Abstract