Scholarlink

<p>The development of cost-effective nanozymes with signal amplification function to overcome the limited sensitivity of lateral flow immunoassays (LFIAs) is of great significance. Here, we introduce the first Mn<sub>2</sub>O<sub>3</sub>-based colorimetric LFIA platform for sensitive C-reactive protein (CRP) detection. By confining Mn<sub>2</sub>O<sub>3</sub> within dendritic mesoporous silica nanoparticles (DMSNs), DMSN-Mn<sub>2</sub>O<sub>3</sub> nanozymes have been synthesized with enhanced active site exposure and uniform particle sizes. It is demonstrated that DMSN-Mn<sub>2</sub>O<sub>3</sub> exhibits better catalytic performance compared to DMSN-MnO<sub>2</sub> and pure Mn<sub>2</sub>O<sub>3</sub>, showing the importance of control over both composition and nanostructure in enhancing the nanozyme functions. In the built H<sub>2</sub>O<sub>2</sub>-free colorimetric LFIA platform, DMSN-Mn<sub>2</sub>O<sub>3</sub> demonstrates rapid catalytic amplification (30&#xa0;s) for CRP detection, achieving a limit of detection of 0.25 ng mL<sup>−1</sup>, a 6-fold improvement over conventional colloidal gold-based LFIAs. This work contributes to the rational design of high-performance nanozymes and provides a platform technology for point-of-care diagnostics with enhanced sensitivity.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Oxidase activity-enhanced manganese (III) oxide nanozyme grown in dendritic mesoporous silica nanoparticles for sensitive lateral flow immunoassay of C-reactive protein

  • 期刊论文

  • 发表日期：

    2026年1月10日

查看全文
  • Jiaqi Fang,
  • Fang Gao,
  • Ye Zhang,
  • Yining Yao,
  • Shaonian Ye,
  • Chao Liu,
  • Chengzhong Yu

摘要

The development of cost-effective nanozymes with signal amplification function to overcome the limited sensitivity of lateral flow immunoassays (LFIAs) is of great significance. Here, we introduce the first Mn2O3-based colorimetric LFIA platform for sensitive C-reactive protein (CRP) detection. By confining Mn2O3 within dendritic mesoporous silica nanoparticles (DMSNs), DMSN-Mn2O3 nanozymes have been synthesized with enhanced active site exposure and uniform particle sizes. It is demonstrated that DMSN-Mn2O3 exhibits better catalytic performance compared to DMSN-MnO2 and pure Mn2O3, showing the importance of control over both composition and nanostructure in enhancing the nanozyme functions. In the built H2O2-free colorimetric LFIA platform, DMSN-Mn2O3 demonstrates rapid catalytic amplification (30 s) for CRP detection, achieving a limit of detection of 0.25 ng mL−1, a 6-fold improvement over conventional colloidal gold-based LFIAs. This work contributes to the rational design of high-performance nanozymes and provides a platform technology for point-of-care diagnostics with enhanced sensitivity.

Graphical Abstract