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<p>A confined plasmonic colorimetric multisensor adapted microplate format was developed to detect in vitro H<sub>2</sub>S released by cardiomyocytes. The device is based on nylon membranes spotted with citrate- capped AgNPs and allows configuration of 24, 48 or 96 sensor spots. Upon exposure to H₂S at ppb levels, AgNPs change color from yellow to brown as the plasmonic band shifts to higher wavelengths, correlating with H₂S concentration. The multisensor and the experimental conditions were optimized to meet the requirements of this biological application including sensitivity, exposure times and cell number. Simulated clinical conditions were tested in AC10 cardiomyocytes and iPSC-derived cardiomyocytes. The method was validated for 24-well plates, achieving a limit of quantification (LOQ) between 0.025 and 0.029&#xa0;µg H₂S for exposure times ranging from 2 to 20&#xa0;h, with a relative standard deviation (RSD) below 15%. Satisfactory results were obtained in the determination of H<sub>2</sub>S released by iPSCs and AC10 cardiomyocytes, with observed levels of approximately 0.4&#xa0;µg of H<sub>2</sub>S. According to these results, it can be concluded that the developed confined multisensor system is suitable for multiple in situ monitoring of H<sub>2</sub>S released by cardiomyocytes in vitro. Additionally, a quantitative evaluation of sustainability was performed using the hexagon tool.</p> Graphical Abstract <p></p>

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Confined plasmonic device for green and sustainable determination of endogenous H2S emitted by living cells: application to cardiomyocytes

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Lusine Hakobyan,
  • Ivan Carrero-Ferrer,
  • Ana Ballester-Caudet,
  • Marta Gómez-Ferrer,
  • Imelda Ontoria-Oviedo,
  • Akaitz Dorronsoro,
  • Sandra Tejedor-Gascón,
  • Pilar Sepúlveda,
  • Carmen Molins-Legua,
  • Pilar Campíns-Falcó

摘要

A confined plasmonic colorimetric multisensor adapted microplate format was developed to detect in vitro H2S released by cardiomyocytes. The device is based on nylon membranes spotted with citrate- capped AgNPs and allows configuration of 24, 48 or 96 sensor spots. Upon exposure to H₂S at ppb levels, AgNPs change color from yellow to brown as the plasmonic band shifts to higher wavelengths, correlating with H₂S concentration. The multisensor and the experimental conditions were optimized to meet the requirements of this biological application including sensitivity, exposure times and cell number. Simulated clinical conditions were tested in AC10 cardiomyocytes and iPSC-derived cardiomyocytes. The method was validated for 24-well plates, achieving a limit of quantification (LOQ) between 0.025 and 0.029 µg H₂S for exposure times ranging from 2 to 20 h, with a relative standard deviation (RSD) below 15%. Satisfactory results were obtained in the determination of H2S released by iPSCs and AC10 cardiomyocytes, with observed levels of approximately 0.4 µg of H2S. According to these results, it can be concluded that the developed confined multisensor system is suitable for multiple in situ monitoring of H2S released by cardiomyocytes in vitro. Additionally, a quantitative evaluation of sustainability was performed using the hexagon tool.

Graphical Abstract