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<p> An electrochemical immunosensor was developed based on silver-ruthenium nanoparticles functionalized NH<sub>2</sub>-MIL-88B (NH<sub>2</sub>-MIL-88B/Ag@Ru) composites for sensitive detection of cardiac troponin I (cTnI). The NH<sub>2</sub>-MIL-88B/Ag@Ru composites shows synergistic peroxidase-like activity with <i>K</i><sub>m</sub> values of 0.737 mM<sup>− 1</sup> for hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and 0.55 mM<sup>− 1</sup> for 3,3’,5,5’-tetramethylbiphenylamine (TMB). Additionally, the electrode surface is modified using a one-step electrodeposition reduction of graphene with chloroauric acid trihydrate (rGO-AuNPs) to increase the effective specific surface area and facilitate charge transfer. By integrating NH<sub>2</sub>-MIL-88B/Ag@Ru composites as a signal amplification tracer and proportionally optimized rGO-AuNPs as the sensing platform, the resulting electrochemical immunosensor exhibits a wide linear detection range from 20&#xa0;fg/mL to 200 ng/mL for cTnI, with a detection limit of 2.15&#xa0;fg/mL. Furthermore, in a doxorubicin (DOX)-induced myocardial injury model, this sensor demonstrated excellent practicality paired with the PalmSens 4 portable analyzer. Detection results were consistent with commercial enzyme-linked immunosorbent assay (ELISA), and serum sample recoveries were excellent. This study provides a feasible strategy for the rapid and sensitive detection of cTnI, demonstrating potential for clinical application.</p> Graphical abstract <p></p>

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An electrochemical immunosensor based on NH2-MIL-88B/Ag@Ru composites for sensitive detection of cardiac troponin I

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • Jianbo Wang,
  • Min Qing,
  • Zhijin Li,
  • Chao Yu

摘要

An electrochemical immunosensor was developed based on silver-ruthenium nanoparticles functionalized NH2-MIL-88B (NH2-MIL-88B/Ag@Ru) composites for sensitive detection of cardiac troponin I (cTnI). The NH2-MIL-88B/Ag@Ru composites shows synergistic peroxidase-like activity with Km values of 0.737 mM− 1 for hydrogen peroxide (H2O2) and 0.55 mM− 1 for 3,3’,5,5’-tetramethylbiphenylamine (TMB). Additionally, the electrode surface is modified using a one-step electrodeposition reduction of graphene with chloroauric acid trihydrate (rGO-AuNPs) to increase the effective specific surface area and facilitate charge transfer. By integrating NH2-MIL-88B/Ag@Ru composites as a signal amplification tracer and proportionally optimized rGO-AuNPs as the sensing platform, the resulting electrochemical immunosensor exhibits a wide linear detection range from 20 fg/mL to 200 ng/mL for cTnI, with a detection limit of 2.15 fg/mL. Furthermore, in a doxorubicin (DOX)-induced myocardial injury model, this sensor demonstrated excellent practicality paired with the PalmSens 4 portable analyzer. Detection results were consistent with commercial enzyme-linked immunosorbent assay (ELISA), and serum sample recoveries were excellent. This study provides a feasible strategy for the rapid and sensitive detection of cTnI, demonstrating potential for clinical application.

Graphical abstract