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<p> A straightforward analysis strategy based on Surface-Enhanced Raman Spectroscopy (SERS) technology was developed for determining amino acid levels in sweat. Polyvinyl butyral (PVB) is an excellent polymer that is used to coat the surface of sulfonated cellulose silver nanocomposites (S-CNF-Ag NPs) films to improve the mechanical properties and sensing properties of the two-dimensional substrate. The S-CNF-Ag-PVB film showed a significant increase in tensile stress and strain, reaching a stress level of 88.34&#xa0;MPa, toughness of 5620 kJ/m<sup>3</sup>, and a 3-fold increase in strain. It also exhibits excellent SERS activity when detecting methylene blue (MB) signal molecules, with a minimum detection of 10<sup>−9</sup> M. As an ultrasensitive SERS sensor, S-CNF-Ag-PVB demonstrates excellent sensing properties for tryptophan (Trp), tyrosine (Tyr), arginine (Arg), glutamic acid (Glu), and phenylalanine (Phe) as sweat metabolites. The developed two-dimensional substrate has been effectively utilized for the precise detection of amino acids in sweat.</p> Graphical Abstract <p></p>

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A sulfonated nanocellulose-silver composite two-dimensional substrate ​​for SERS detection of​​ various amino acids in sweat

  • 期刊论文

  • 发表日期：

    2025年11月29日

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  • Jinglei Yang,
  • Wenxi Wang,
  • Peipei Xu,
  • Yeting Tu,
  • Ruiyun You,
  • Yuhua Hu,
  • Qingqiang Lin,
  • Yudong Lu

摘要

A straightforward analysis strategy based on Surface-Enhanced Raman Spectroscopy (SERS) technology was developed for determining amino acid levels in sweat. Polyvinyl butyral (PVB) is an excellent polymer that is used to coat the surface of sulfonated cellulose silver nanocomposites (S-CNF-Ag NPs) films to improve the mechanical properties and sensing properties of the two-dimensional substrate. The S-CNF-Ag-PVB film showed a significant increase in tensile stress and strain, reaching a stress level of 88.34 MPa, toughness of 5620 kJ/m3, and a 3-fold increase in strain. It also exhibits excellent SERS activity when detecting methylene blue (MB) signal molecules, with a minimum detection of 10−9 M. As an ultrasensitive SERS sensor, S-CNF-Ag-PVB demonstrates excellent sensing properties for tryptophan (Trp), tyrosine (Tyr), arginine (Arg), glutamic acid (Glu), and phenylalanine (Phe) as sweat metabolites. The developed two-dimensional substrate has been effectively utilized for the precise detection of amino acids in sweat.

Graphical Abstract