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<p>Infectious pneumonia represents a significant cause of mortality among intensive care unit (ICU) patients. Clinically, the differentiation between viral and bacterial pneumonia has traditionally relied upon sputum culture methodologies, a process requiring approximately 2–3 days. Leveraging nanozyme technology, we have developed a pH-responsive, indirect urease detection platform based on surface-enhanced Raman spectroscopy (SERS). This platform employs Au@Pt nanorods (Au@Pt NRs) as the core catalytic nanozyme. The principle exploits the specific hydrolysis of urea by urease, which subsequently alters the solution pH. This pH shift modulates the catalytic activity of the nanozyme, thereby influencing the production rate of oxidized 3,3’,5,5’-tetramethylbenzidine (ox-TMB). The resultant change in ox-TMB concentration directly impacts the generated SERS signal intensity. The entire assay can be completed within 25&#xa0;min, demonstrating exceptional rapidity. The platform provides high sensitivity, with a detection limit as low as 10.44 U·L<sup>−1</sup>. Furthermore, the platform demonstrates excellent reproducibility, stability, specificity, and robust peroxidase-like activity. Validation using clinical samples confirmed the high accuracy of this detection platform. These findings indicate that this method holds considerable promise for the rapid, straightforward, and sensitive distinction between bacterial and viral pneumonia.</p>

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A pH-responsive nanozymes Au@Pt NRs SERS assay platform for the detection of urease enables efficient and accurate differentiation of bacterial and viral pneumonia

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Ruoyu Zhou,
  • Xudong Zhang,
  • Yongli Wu,
  • Tianran Li,
  • Dongxu Zhu,
  • Qiong Xu,
  • Yayun Qian,
  • Taijing Xu

摘要

Infectious pneumonia represents a significant cause of mortality among intensive care unit (ICU) patients. Clinically, the differentiation between viral and bacterial pneumonia has traditionally relied upon sputum culture methodologies, a process requiring approximately 2–3 days. Leveraging nanozyme technology, we have developed a pH-responsive, indirect urease detection platform based on surface-enhanced Raman spectroscopy (SERS). This platform employs Au@Pt nanorods (Au@Pt NRs) as the core catalytic nanozyme. The principle exploits the specific hydrolysis of urea by urease, which subsequently alters the solution pH. This pH shift modulates the catalytic activity of the nanozyme, thereby influencing the production rate of oxidized 3,3’,5,5’-tetramethylbenzidine (ox-TMB). The resultant change in ox-TMB concentration directly impacts the generated SERS signal intensity. The entire assay can be completed within 25 min, demonstrating exceptional rapidity. The platform provides high sensitivity, with a detection limit as low as 10.44 U·L−1. Furthermore, the platform demonstrates excellent reproducibility, stability, specificity, and robust peroxidase-like activity. Validation using clinical samples confirmed the high accuracy of this detection platform. These findings indicate that this method holds considerable promise for the rapid, straightforward, and sensitive distinction between bacterial and viral pneumonia.