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<p>A novel immunomagnetic bead was prepared and designed to be combined with a smartphone app for the rapid detection of SARS-CoV-2 antibody in serum. The nucleocapsid protein (N protein) of SARS-CoV-2 was immobilized on the prepared magnetic polymer microspheres (Fe<sub>3</sub>O<sub>4</sub>@PMAA) with core-shell structure by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimid/N-hydroxy succinimide (EDC/NHS) to form the immunomagnetic beads (Fe<sub>3</sub>O<sub>4</sub>@PMAA-NP) capable of binding antibodies specifically. Combining its magnetic separability with the image analysis function of the smartphone app, a novel detection platform was established on a 96-well plate. The platform can realize rapid separation and colorimetric detection of antibodies within 30&#xa0;min, and the detection limit is 0.5&#xa0;µg/mL. This method has the advantages of portability, rapidity, high sensitivity and low cost, which is suitable for home self-test and on-site detection. This study not only provides an innovative method for the antibody detection of SARS-CoV-2 in serum, but also provides a favorable reference for the practical application of functionalized magnetic polymer materials in the medical field.</p> Graphical Abstract <p></p>

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Preparation of Fe3O4@PMAA-N protein magnetic beads for antibody detection of SARS-CoV-2 based on indirect ELISA smartphone visual strategy

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • Zheng Nie,
  • Juan Sun,
  • Fangjun Wu,
  • Fujie Lai,
  • Xudong Yang,
  • Shaoyang Chen,
  • Yang Wang,
  • Yong Jiang

摘要

A novel immunomagnetic bead was prepared and designed to be combined with a smartphone app for the rapid detection of SARS-CoV-2 antibody in serum. The nucleocapsid protein (N protein) of SARS-CoV-2 was immobilized on the prepared magnetic polymer microspheres (Fe3O4@PMAA) with core-shell structure by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimid/N-hydroxy succinimide (EDC/NHS) to form the immunomagnetic beads (Fe3O4@PMAA-NP) capable of binding antibodies specifically. Combining its magnetic separability with the image analysis function of the smartphone app, a novel detection platform was established on a 96-well plate. The platform can realize rapid separation and colorimetric detection of antibodies within 30 min, and the detection limit is 0.5 µg/mL. This method has the advantages of portability, rapidity, high sensitivity and low cost, which is suitable for home self-test and on-site detection. This study not only provides an innovative method for the antibody detection of SARS-CoV-2 in serum, but also provides a favorable reference for the practical application of functionalized magnetic polymer materials in the medical field.

Graphical Abstract