Scholarlink

<p>A&#xa0;multifunctional polydopamine-gold (PDA@Au) core–shell nanoprobe has been synthesized&#xa0;that simultaneously enables surface-enhanced Raman scattering (SERS), photothermal conversion, and visual signal output within a single structure. Based on these features, we developed a dual-modal LFA platform for H1N1 antigens detection through two complementary readout modes. The SERS and photothermal modes achieved detection limits of 1.34&#xa0;pg/mL and 1.03&#xa0;pg/mL, corresponding to 746-fold and 971-fold improvements compared to commercial Au-based LFAs. The platform also exhibited excellent specificity against other common respiratory viruses and high batch-to-batch reproducibility across detection modes. Clinical validation on 90 throat swab specimens showed strong agreement with RT-PCR results. The PDA@Au-LFA demonstrated sensitivities of 98.5% (SERS), 97.0% (photothermal), and 93.9% (visual), along with consistently high specificity (95.8%). These results highlight the PDA@Au-based LFA as a high-performance diagnostic platform, featuring sensitive, specific, and reproducible multimodal detection enabled by the integration of a novel signal transduction material.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated SERS and photothermal dual-mode ultrasensitive immunochromatographic detection for influenza A based on PDA@Au nanoprobes

  • 期刊论文

  • 发表日期：

    2025年11月10日

查看全文
  • Renlong Zhu,
  • Jing Liang,
  • Zhenzhen Liu,
  • Xiaolan Huang,
  • Xiaofei Jia,
  • MengKe Fang,
  • Yi Wang,
  • Rui Xiao,
  • Yajie Wang

摘要

A multifunctional polydopamine-gold (PDA@Au) core–shell nanoprobe has been synthesized that simultaneously enables surface-enhanced Raman scattering (SERS), photothermal conversion, and visual signal output within a single structure. Based on these features, we developed a dual-modal LFA platform for H1N1 antigens detection through two complementary readout modes. The SERS and photothermal modes achieved detection limits of 1.34 pg/mL and 1.03 pg/mL, corresponding to 746-fold and 971-fold improvements compared to commercial Au-based LFAs. The platform also exhibited excellent specificity against other common respiratory viruses and high batch-to-batch reproducibility across detection modes. Clinical validation on 90 throat swab specimens showed strong agreement with RT-PCR results. The PDA@Au-LFA demonstrated sensitivities of 98.5% (SERS), 97.0% (photothermal), and 93.9% (visual), along with consistently high specificity (95.8%). These results highlight the PDA@Au-based LFA as a high-performance diagnostic platform, featuring sensitive, specific, and reproducible multimodal detection enabled by the integration of a novel signal transduction material.

Graphical Abstract