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<p>A&#xa0;core–shell composite nanomaterial PAu@Ag with structural stability was developed by integrating a layer of Au@Ag bimetallic nanoparticles (NPs) on the polydopamine (PDA) surface. The synergy between PDA and Au@Ag with localized surface plasmon resonance (LSPR) improved the colorimetric performance; simultaneously, the nanogap-arranged Au@Ag NPs created a high density of “hotspots”, resulting in markedly enhanced SERS activity. The multiplex lateral flow immunoassay&#xa0;(LFIA), based on PAu@Ag colorimetric/SERS dual-enhanced tags, featuring dual-mode detection of rapid qualitative screening (colorimetric) and ultrasensitive quantitative analysis (SERS), demonstrated excellent performance in simultaneously detecting two highly transmissible respiratory viruses. The colorimetric and SERS enhanced results were 2 to more than 750 times more sensitive than the non-integrated method (Au@Ag − LFIA) and commercial kits (Au − LFIA and ELISA), along with 100% accuracy in 36 clinical samples detection, making PAu@Ag − LFIA show great potential as a universal and flexible immunodiagnostic of viruses.</p> Graphical Abstract <p></p>

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PDA-integrated Au@Ag with colorimetric/SERS dual-signal enhancement capability for flexible lateral flow immunoassay detection of respiratory viruses

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • Xiaoxian Liu,
  • Zhenyong Dong,
  • Hao Wang,
  • Guanghui Wang,
  • Rui Xiao

摘要

A core–shell composite nanomaterial PAu@Ag with structural stability was developed by integrating a layer of Au@Ag bimetallic nanoparticles (NPs) on the polydopamine (PDA) surface. The synergy between PDA and Au@Ag with localized surface plasmon resonance (LSPR) improved the colorimetric performance; simultaneously, the nanogap-arranged Au@Ag NPs created a high density of “hotspots”, resulting in markedly enhanced SERS activity. The multiplex lateral flow immunoassay (LFIA), based on PAu@Ag colorimetric/SERS dual-enhanced tags, featuring dual-mode detection of rapid qualitative screening (colorimetric) and ultrasensitive quantitative analysis (SERS), demonstrated excellent performance in simultaneously detecting two highly transmissible respiratory viruses. The colorimetric and SERS enhanced results were 2 to more than 750 times more sensitive than the non-integrated method (Au@Ag − LFIA) and commercial kits (Au − LFIA and ELISA), along with 100% accuracy in 36 clinical samples detection, making PAu@Ag − LFIA show great potential as a universal and flexible immunodiagnostic of viruses.

Graphical Abstract