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<p> A&#xa0;novel dual-mode sensing system integrating a magnetic core–shell&#xa0;CuFe<sub>2</sub>O<sub>4</sub>/Cu/MnO<sub>2</sub> nanozyme&#xa0;with a stimuli-responsive&#xa0;agarose-deep eutectic solvent hydrogel (DES-Aga)&#xa0;is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (K<sub>m</sub> = 0.14&#xa0;mM) and high catalytic efficiency (V<sub>max</sub> = 1.89 × 10<sup>−6</sup>&#xa0;M·s<sup>−1</sup>), enabling rapid oxidation of TMB to generate a colorimetric signal. Coupled with the DES-Aga hydrogel, the platform achieves dual-mode detection: laboratory-grade UV–vis quantification (detection limits: 0.01&#xa0;μM HQ, 0.05&#xa0;μM GSH, 0.02&#xa0;μM NO<sub>2</sub><sup>−</sup>) and smartphone-assisted on-site analysis. The hydrogel leverages redox/diazotization interactions to produce distinct color transitions (blue → colorless for HQ/GSH; blue → yellow for NO<sub>2</sub><sup>−</sup>), validated in real-world matrices (cosmetics, food, serum) with recoveries of 87–115% and RSD &lt; 8.6%. Key innovations include the nanozyme’s magnetic recyclability (&gt; 80% activity after 7 cycles), the hydrogel’s stability (&gt; 90% retention after 7&#xa0;days), and a ratiometric strategy for NO<sub>2</sub><sup>−</sup>&#xa0;detection. This work bridges the gap between laboratory precision and field-deployable diagnostics, offering a versatile tool for monitoring carcinogens in consumer products, food contaminants, and oxidative stress biomarkers, with direct implications for public health and safety.</p> Graphical abstract <p></p>

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Dual-mode nanozyme-hydrogel platform for on-site multi-target detection of biomarkers and hazardous substances

  • 期刊论文

  • 发表日期：

    2025年9月8日

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  • Junjie Zhao,
  • Jiawei Hong,
  • Haonan Li,
  • Haonan Zhang,
  • Longshan Zhao

摘要

A novel dual-mode sensing system integrating a magnetic core–shell CuFe2O4/Cu/MnO2 nanozyme with a stimuli-responsive agarose-deep eutectic solvent hydrogel (DES-Aga) is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (Km = 0.14 mM) and high catalytic efficiency (Vmax = 1.89 × 10−6 M·s−1), enabling rapid oxidation of TMB to generate a colorimetric signal. Coupled with the DES-Aga hydrogel, the platform achieves dual-mode detection: laboratory-grade UV–vis quantification (detection limits: 0.01 μM HQ, 0.05 μM GSH, 0.02 μM NO2−) and smartphone-assisted on-site analysis. The hydrogel leverages redox/diazotization interactions to produce distinct color transitions (blue → colorless for HQ/GSH; blue → yellow for NO2−), validated in real-world matrices (cosmetics, food, serum) with recoveries of 87–115% and RSD < 8.6%. Key innovations include the nanozyme’s magnetic recyclability (> 80% activity after 7 cycles), the hydrogel’s stability (> 90% retention after 7 days), and a ratiometric strategy for NO2− detection. This work bridges the gap between laboratory precision and field-deployable diagnostics, offering a versatile tool for monitoring carcinogens in consumer products, food contaminants, and oxidative stress biomarkers, with direct implications for public health and safety.

Graphical abstract