Scholarlink

<p>A&#xa0;label-free hybridization chain reaction (HCR)–facilitated nanozyme catalysis and fluorescence simultaneous amplification strategy is proposed&#xa0;for the ultrasensitive fluorescence-colorimetric dual-mode detection of <i>S. typhimurium</i> in complex food matrices. In the approach, aptamer-functionalized magnetic beads specifically captured <i>S. typhimurium</i>, and the cDNA triggered HCR to generate long and rigid double-stranded DNAs (dsDNAs), which could be inserted with SYBR Green I (SGI) and regulate the surface charge and spatial distribution of gold nanoparticles (AuNPs). Consequently, the produced dsDNAs simultaneously enhanced the fluorescence intensity of SYBR Green I and the enzyme-like activity of AuNPs catalyzing the 3,3′,5,5′-tetramethylbenzidine chromogenic reaction, yielding robust dual-signal outputs for quantification. With the label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification feature, the detection Limit of our dual-mode system is as low as 1&#xa0;CFU/mL, and its analytical application in real food samples was also verified with satisfactory accuracy. The proposed HCR-enabled sensing system furnishes an ultrasensitive and precise method for the detection of foodborne pathogens, holding great application potential in food safety analysis and other fields.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification for ultrasensitive dual-signal sensing of foodborne Salmonella typhimurium

  • 期刊论文

  • 发表日期：

    2025年9月15日

查看全文
  • Fengxia Zhao,
  • Shiyu Jia,
  • Hangli Yan,
  • Qinqin Bai,
  • Hongmei Hu,
  • Hao Liang,
  • Xiangheng Niu

摘要

A label-free hybridization chain reaction (HCR)–facilitated nanozyme catalysis and fluorescence simultaneous amplification strategy is proposed for the ultrasensitive fluorescence-colorimetric dual-mode detection of S. typhimurium in complex food matrices. In the approach, aptamer-functionalized magnetic beads specifically captured S. typhimurium, and the cDNA triggered HCR to generate long and rigid double-stranded DNAs (dsDNAs), which could be inserted with SYBR Green I (SGI) and regulate the surface charge and spatial distribution of gold nanoparticles (AuNPs). Consequently, the produced dsDNAs simultaneously enhanced the fluorescence intensity of SYBR Green I and the enzyme-like activity of AuNPs catalyzing the 3,3′,5,5′-tetramethylbenzidine chromogenic reaction, yielding robust dual-signal outputs for quantification. With the label-free HCR-facilitated nanozyme catalysis and fluorescence simultaneous amplification feature, the detection Limit of our dual-mode system is as low as 1 CFU/mL, and its analytical application in real food samples was also verified with satisfactory accuracy. The proposed HCR-enabled sensing system furnishes an ultrasensitive and precise method for the detection of foodborne pathogens, holding great application potential in food safety analysis and other fields.

Graphical abstract