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<p>Pyocyanin (PYO) is a unique electroactive virulence factor secreted by <i>Pseudomonas aeruginosa</i>, a pathogen responsible for various severe infections, especially in critically ill patients. Given its redox-active nature, PYO is well-suited for electrochemical detection. In this study, a nanocomposite of tungsten disulfide nanosheets supported by nanodiamonds (WS<sub>2</sub> NSs–ND) was physically cross-linked onto a glassy carbon electrode (GCE) to enhance sensitivity and electron transfer. The WS<sub>2</sub> NSs–ND-modified electrode exhibited a high sensitivity of 0.38 µA µM<sup>−1</sup>&#xa0;cm<sup>−2</sup> due to its increased active surface area. The sensor also demonstrated excellent performance in real human urine samples, with a relative standard deviation (RSD) of ± 2%, confirming its reliability and potential for clinical application in the rapid detection of PYO.</p> Graphical Abstract <p></p>

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Nanodiamond-embedded tungsten disulfide nanosheets for electrochemical detection of a bacterial metabolite pyocyanin

  • 期刊论文

  • 发表日期：

    2025年8月25日

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  • Shanmuganathan Keerthana,
  • Karutha Pandian Divya,
  • A. Rajapriya,
  • Chinnuswamy Viswanathan,
  • Nagamony Ponpandian

摘要

Pyocyanin (PYO) is a unique electroactive virulence factor secreted by Pseudomonas aeruginosa, a pathogen responsible for various severe infections, especially in critically ill patients. Given its redox-active nature, PYO is well-suited for electrochemical detection. In this study, a nanocomposite of tungsten disulfide nanosheets supported by nanodiamonds (WS2 NSs–ND) was physically cross-linked onto a glassy carbon electrode (GCE) to enhance sensitivity and electron transfer. The WS2 NSs–ND-modified electrode exhibited a high sensitivity of 0.38 µA µM−1 cm−2 due to its increased active surface area. The sensor also demonstrated excellent performance in real human urine samples, with a relative standard deviation (RSD) of ± 2%, confirming its reliability and potential for clinical application in the rapid detection of PYO.

Graphical Abstract