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<p>Nanobodies specifically recognizing atrazine were successfully obtained through immunization of alpacas and phage display libraries, and an indirect competitive immunoassay (ic-ELISA) based on nanobodies was constructed with an IC<sub>50</sub> of 0.062&#xa0;μg/mL and a minimum detection limit of 0.01&#xa0;μg/mL. An&#xa0;Au@PtNPs with excellent colorimetric performance, high catalytic activity, and high stability was synthesized. The combination of Au@PtNPs and nanobodies led to the development of a direct competitive immunoassay (dc-ELISA) with higher specificity, convenience, lower cost and an IC<sub>50</sub> value of 0.032&#xa0;μg/mL which was lower than the ic-ELISA. Based on the Au@PtNPs and nanobodies, a novel LFIA model for qualitative and semi-quantitative detection of atrazine was constructed with a visual detection limit of 5&#xa0;ng/mL. The present study is of great significance for the construction of atrazine immunoassay combining nanobodies and nanoenzymes, which provides a sensitive, specific, simple and reliable method for the detection of atrazine in the environment.</p> Graphical Abstract <p></p>

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Development of an atrazine immunoassay based on highly catalytically active Au@PtNPs and specific nanobody

  • 期刊论文

  • 发表日期：

    2025年8月15日

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  • Jia Kang,
  • Meng Qi,
  • Yasen Wang,
  • Linchun Li,
  • Xinda Zhou,
  • Xiaoshuo An,
  • Bruce D. Hammock,
  • Jinlin Zhang,
  • Jingqian Huo

摘要

Nanobodies specifically recognizing atrazine were successfully obtained through immunization of alpacas and phage display libraries, and an indirect competitive immunoassay (ic-ELISA) based on nanobodies was constructed with an IC50 of 0.062 μg/mL and a minimum detection limit of 0.01 μg/mL. An Au@PtNPs with excellent colorimetric performance, high catalytic activity, and high stability was synthesized. The combination of Au@PtNPs and nanobodies led to the development of a direct competitive immunoassay (dc-ELISA) with higher specificity, convenience, lower cost and an IC50 value of 0.032 μg/mL which was lower than the ic-ELISA. Based on the Au@PtNPs and nanobodies, a novel LFIA model for qualitative and semi-quantitative detection of atrazine was constructed with a visual detection limit of 5 ng/mL. The present study is of great significance for the construction of atrazine immunoassay combining nanobodies and nanoenzymes, which provides a sensitive, specific, simple and reliable method for the detection of atrazine in the environment.

Graphical Abstract