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<p>The overuse of tetracycline (TC) a&#xa0;novel reverse ELISA assay integrating quantum dots (QDs) with an allosteric transcription factor (TetR) was developed for rapid tetracycline (TC) detection. Specifically, biotin-modified double-stranded DNA (dsDNA) was immobilized on a streptavidin-coated 96-well plate, after which QD-TetR conjugates were added. In the presence of TC, the QD-TetR conjugate binds TC and undergoes allosteric changes that result in its dissociation from the dsDNA. The developed QD-TetR-based reverse ELISA achieved quantitative TC detection within a linear dynamic range of 0.05–100&#xa0;μM in just 5&#xa0;min, with a method detection limit of 0.02&#xa0;μM. The recoveries ranged from 93.43% to 106.6% for actual samples (seawater, sewage, milk, and honey) detection. Furthermore, on-site semi-quantitative detection of TC in actual samples was achieved across a concentration range 0.5–500&#xa0;μM. The results were validated with commercial ELISA kits, indicating strong potential for monitoring TC residues in environmental and food samples.</p> Graphical abstract <p></p>

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A reverse ELISA based on quantum dot-labeled TetR for rapid detection of tetracycline

  • 期刊论文

  • 发表日期：

    2025年8月12日

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  • Huaixiu Bi,
  • Cheng Zhang,
  • Chen Zhao,
  • Xi Zhang,
  • Chenyu Li,
  • Shang Wang,
  • Xiaobo Yang,
  • Zhigang Qiu,
  • Jingfeng Wang,
  • Bin Xue,
  • Zhiqiang Shen

摘要

The overuse of tetracycline (TC) a novel reverse ELISA assay integrating quantum dots (QDs) with an allosteric transcription factor (TetR) was developed for rapid tetracycline (TC) detection. Specifically, biotin-modified double-stranded DNA (dsDNA) was immobilized on a streptavidin-coated 96-well plate, after which QD-TetR conjugates were added. In the presence of TC, the QD-TetR conjugate binds TC and undergoes allosteric changes that result in its dissociation from the dsDNA. The developed QD-TetR-based reverse ELISA achieved quantitative TC detection within a linear dynamic range of 0.05–100 μM in just 5 min, with a method detection limit of 0.02 μM. The recoveries ranged from 93.43% to 106.6% for actual samples (seawater, sewage, milk, and honey) detection. Furthermore, on-site semi-quantitative detection of TC in actual samples was achieved across a concentration range 0.5–500 μM. The results were validated with commercial ELISA kits, indicating strong potential for monitoring TC residues in environmental and food samples.

Graphical abstract