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<p> A&#xa0;nano-theranostic system&#xa0;is reported that integrates imaging, detection, and treatment. The DNA assembly self-assembled by rolling circle amplification (RCA) product and four oligonucleotides are combined with MnO₂ nanosheets to construct DNA assembly@MnO₂. One repeat unit of the DNA assembly contains an aptamer module for specific targeting, a metal ion-activated intracellular imaging module, and a DNAzyme module for gene therapy (GT). Upon entering cancer cells, MnO₂ nanosheets are reduced to Mn<sup>2</sup>⁺ by the high intracellular concentration of glutathione (GSH). On the one hand, Mn<sup>2</sup>⁺ serves as a chemodynamic therapeutic agent to trigger Fenton-like reactions for chemodynamic therapy (CDT), and on the other hand, it can serve as a metal cofactor to activate the DNAzyme. This nanosystem can not only image specific cancer cells but also sensitively detect MCF-7 cells with a detection limit of 341 cells/mL. Moreover, this system can perform gene-chemodynamic combination therapy and has great application potential in cancer diagnosis and treatment.</p> Graphical abstract <p></p>

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An all-in-one MnO₂-DNA assembly nanoplatform for imaging, detection, and gene-chemodynamic combination therapy of MCF-7 cells

  • 期刊论文

  • 发表日期：

    2025年5月31日

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  • Ruyan Zhang,
  • Zhiqing Zhang,
  • Guodong Zhang,
  • Jingyun Zhuang,
  • Xiaobing Huo,
  • Gan Ning,
  • Tianxin Weng,
  • Xiufeng Wang,
  • Ting Zhou,
  • Fang Wang

摘要

A nano-theranostic system is reported that integrates imaging, detection, and treatment. The DNA assembly self-assembled by rolling circle amplification (RCA) product and four oligonucleotides are combined with MnO₂ nanosheets to construct DNA assembly@MnO₂. One repeat unit of the DNA assembly contains an aptamer module for specific targeting, a metal ion-activated intracellular imaging module, and a DNAzyme module for gene therapy (GT). Upon entering cancer cells, MnO₂ nanosheets are reduced to Mn2⁺ by the high intracellular concentration of glutathione (GSH). On the one hand, Mn2⁺ serves as a chemodynamic therapeutic agent to trigger Fenton-like reactions for chemodynamic therapy (CDT), and on the other hand, it can serve as a metal cofactor to activate the DNAzyme. This nanosystem can not only image specific cancer cells but also sensitively detect MCF-7 cells with a detection limit of 341 cells/mL. Moreover, this system can perform gene-chemodynamic combination therapy and has great application potential in cancer diagnosis and treatment.

Graphical abstract