<p>T-2 toxin, a mycotoxin commonly found in food, is recognized as one of the most harmful contaminants. Herein, a sensitive photoelectrochemical (PEC) aptasensor based on an “on–off-on” signal response strategy was developed for T-2 detection using WO₃/CdIn₂S₄ as a photoanode. The sensitization of WO₃ with CdIn₂S₄ significantly enhanced the photocurrent, leading to the initial “signal-on” state. As a signal label of probe DNA (pDNA), CoO significantly inhibited the photocurrent response of WO₃/CdIn₂S₄ (“signal-off” state), enhancing the signal recovery space and improving the detection sensitivity. T-2 toxin is&#xa0;preferentially bound to aDNA, releasing the CoO-pDNA complex from the electrode, realizing the “signal-on” state again. This signal switching mechanism enabled a broad detection range from 1&#xa0;fg&#xa0;mL^–1 to 1&#xa0;μg&#xa0;mL^–1 with an ultralow detection limit of 0.434&#xa0;fg&#xa0;mL^–1 (S/N = 3), while the sensor exhibited excellent reproducibility, stability and selectivity. This platform not only provided a robust analytical tool for T-2 toxin detection in food safety but also established a generalizable sensing paradigm adaptable to other mycotoxins by replacing the recognition element.</p> Graphical Abstract <p></p>