<p>To further explore the boron-involved nanomaterials toward efficient applications in chemo/bio sensing and detection fields, this work reports facile preparation of the emerging iron/cobalt co-doped boron quantum dots (Fe/Co@BQDs) that were explored as new artificial nanozymes for ratiometric fluorescence (FL) and visual detection of glutamate (Glu). In the presence of glutamate oxidase (GLOD), Glu was oxidized to produce H₂O₂, and then the H₂O₂ was catalyzed by Fe/Co@ BQDs nanozymes to produce hydroxyl radical (•OH). Afterwards, the •OH induced FL quenching responses of rhodamine B (RhB) and Fe/Co@BQDs. Therefore, a new nanozyme-assisted cascade catalysis platform was explored, consisting of Fe/Co@BQDs, GLOD, and RhB. The platform was successfully used for ratiometric FL sensing of Glu and liquid/solid dual-channel FL visual semi-quantitative detection of Glu. The platform exhibits a board linear detection range of 1–500&#xa0;µM, a low limit of detection of 0.3&#xa0;µM, highly selective ratiometric FL responses on Glu over potential interferents, and high-performance practical detection of Glu in biological samples. Experimental results verify high peroxidase-like activities of Fe/Co@BQDs that enable efficient applications for unique enzymatic reactions and nanozyme-involved cascade catalysis reactions. The platform can facilitate further development of other types of metal-doped nanomaterials with natural biological enzyme-like activities and their promising applications, especially chemo/bio sensing, bioimaging and therapeutics at the levels of living cells and small animals.</p> Graphical Abstract <p></p>