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<p> A&#xa0;new nanoparticles-based sensing platform was designed for high-through electrochemical immunoassay of ferritin (FET) biomarker on a magneto-controlled microfluidic device by using anti-FET capture antibody-conjugated magnetic sensing probes. Thionine-doped calcium carbonate (CaCO<sub>3</sub>) nanoparticles labeled with anti-FET detection antibodies were utilized as the recognition elements. Introduction of target FET caused the sandwich-type immunoreaction between two antibodies. The formed immunocomplexes were attached onto magnetic microfluidic sensing interface through an external magnet. Subsequently, the carried CaCO<sub>3</sub> nanoparticles were dissolved under acidic conditions to release the doped thionine molecules with redox activity. The thionine-based voltammetric signals increased with the increment of target FET levels within the linear range 0.01–100 ng mL<sup>−1</sup>. The limit of detection was 7.9 pg mL<sup>−1</sup> FET. Good analytical properties such as selectivity, reproducibility, and accuracy were achieved with the&#xa0;nanoparticles-based magnetic electrochemical immunoassay. More significantly, the&#xa0;magnetic microfluidic electrochemical immunoassay provides new opportunities for rapid, simple, and&#xa0;cost-effective serum sample&#xa0;analysis.</p> Graphical Abstract <p></p>

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Nanoparticles-based electrochemical sensing platform for high-through immunoassay with redox-activity CaCO3 nanotags on a magnetic microfluidic device

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Shaofeng Lin,
  • Ting Lin,
  • Haipeng Xu,
  • Haining Li,
  • Wenwen Zhang,
  • Chenyu Wu,
  • Shuyi Lu,
  • Yanping Chen,
  • Xiao Han

摘要

A new nanoparticles-based sensing platform was designed for high-through electrochemical immunoassay of ferritin (FET) biomarker on a magneto-controlled microfluidic device by using anti-FET capture antibody-conjugated magnetic sensing probes. Thionine-doped calcium carbonate (CaCO3) nanoparticles labeled with anti-FET detection antibodies were utilized as the recognition elements. Introduction of target FET caused the sandwich-type immunoreaction between two antibodies. The formed immunocomplexes were attached onto magnetic microfluidic sensing interface through an external magnet. Subsequently, the carried CaCO3 nanoparticles were dissolved under acidic conditions to release the doped thionine molecules with redox activity. The thionine-based voltammetric signals increased with the increment of target FET levels within the linear range 0.01–100 ng mL−1. The limit of detection was 7.9 pg mL−1 FET. Good analytical properties such as selectivity, reproducibility, and accuracy were achieved with the nanoparticles-based magnetic electrochemical immunoassay. More significantly, the magnetic microfluidic electrochemical immunoassay provides new opportunities for rapid, simple, and cost-effective serum sample analysis.

Graphical Abstract