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<p>Multivalent engineering aptamers (multi-Apts) adaptive and specific to whole <i>Escherichia coli</i> (<i>E. coli</i>: CMCC 44102) cells based on hybridization chain reaction (HCR) were constructed&#xa0;for the first time. The dissociation constant (<i>K</i><sub>d</sub>) value for these multi-Apts was 48&#xa0;nM, demonstrating a higher affinity than that of monovalent aptamers (mono-Apts) (<i>K</i><sub>d</sub> = 102&#xa0;nM). Furthermore, the reaction equilibrium of multi-Apts was achieved within 20&#xa0;min, with a reaction rate twice that of mono-Apts. To validate the exceptional performance of these multi-Apts, they were employed as recognition elements in conjunction with gold nanoparticles (AuNPs) colorimetric assays for the all-in-one rapid detection of <i>E. coli</i>. This method exhibited a linear detection range from 1 × 10<sup>2</sup> to 1 × 10⁷ CFU mL⁻<sup>1</sup>, achieving a limit of detection (LOD) as low as 19&#xa0;CFU&#xa0;mL⁻<sup>1</sup>. The recovery of this method in tap water and milk were 85.7% to 101% and 81.8% to 98.2%, respectively. The results indicated that this method not only provided a wide detection range but also exhibited high sensitivity and accuracy. Additionally, this study demonstrated that multi-Apts possessed greater application potential in the detection of macromolecular substances such as bacteria. In short, this work provided a novel approach for rapid and all-in-one detection of <i>E. coli</i> in food.</p> Graphical Abstract <p></p>

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Assembly of adaptive engineering aptamers for Escherichia coli and their application in all-in-one rapid detection

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • Mengyue Liu,
  • Shicai Xu,
  • Giovanna Marrazza

摘要

Multivalent engineering aptamers (multi-Apts) adaptive and specific to whole Escherichia coli (E. coli: CMCC 44102) cells based on hybridization chain reaction (HCR) were constructed for the first time. The dissociation constant (Kd) value for these multi-Apts was 48 nM, demonstrating a higher affinity than that of monovalent aptamers (mono-Apts) (Kd = 102 nM). Furthermore, the reaction equilibrium of multi-Apts was achieved within 20 min, with a reaction rate twice that of mono-Apts. To validate the exceptional performance of these multi-Apts, they were employed as recognition elements in conjunction with gold nanoparticles (AuNPs) colorimetric assays for the all-in-one rapid detection of E. coli. This method exhibited a linear detection range from 1 × 102 to 1 × 10⁷ CFU mL⁻1, achieving a limit of detection (LOD) as low as 19 CFU mL⁻1. The recovery of this method in tap water and milk were 85.7% to 101% and 81.8% to 98.2%, respectively. The results indicated that this method not only provided a wide detection range but also exhibited high sensitivity and accuracy. Additionally, this study demonstrated that multi-Apts possessed greater application potential in the detection of macromolecular substances such as bacteria. In short, this work provided a novel approach for rapid and all-in-one detection of E. coli in food.

Graphical Abstract