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<p> An&#xa0;electrochemical DNA biosensor is presented&#xa0;for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor’s functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identification via genetic code recognition. CNDsTy allow the electrochemical detection based on their different affinity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identification. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples.</p> Graphical Abstract <p></p>

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MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: application in viral infection diagnostics

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月11日

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  • Estefanía Enebral-Romero,
  • Emiliano Martínez-Periñán,
  • David López-Diego,
  • Mónica Luna,
  • Marina Garrido,
  • Cristina Navío,
  • Emilio M. Pérez,
  • Encarnación Lorenzo,
  • Tania García-Mendiola

摘要

An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor’s functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identification via genetic code recognition. CNDsTy allow the electrochemical detection based on their different affinity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identification. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples.

Graphical Abstract