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<p>Despite considerable interest has been shown in research on nanozymes in recent times, the creation of highly active bifunctional nanozymes remains a significant challenge. Inspired by the structural features of the active center of natural laccase, bifunctional copper-based nanozymes (Cu-MB) possessing both laccase-like activity and fluorescence properties using 2-methylimidazole (2-MI) and 2-aminoterephthalic acid (BDC-NH<sub>2</sub>) as ligands&#xa0;have been prepared. The <i>K</i><sub>m</sub> of Cu-MB was 0.0898&#xa0;mM, which was markedly lower than that of previously reported nanozymes and natural laccase. A dual-mode sensor for the detection of epinephrine (EP) was designed based on the high catalytic activity, excellent fluorescence properties, and stability of Cu-MB nanozymes. The limits of detection (LODs) of the colorimetric and fluorescence sensors for EP were 0.22&#xa0;µg/mL and 0.11&#xa0;µg/mL, respectively. The sensor system was verified to have good resistance to interferences and reliability by analyzing actual samples. Furthermore, the integration of a dual-reading hydrogel with a smartphone facilitates the portable, real-time, and rapid assessment of EP. More encouragingly, Cu-MB has excellent substrate versatility and can effectively degrade a diverse array of phenolic pollutants. The developed Cu-MB nanozymes provide a promising approach for the degradation and determination of phenolic compounds. Clinical trial number: not applicable.</p> Graphical abstract <p></p>

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Laccase-mimicking enzyme with fluorescent properties for dual-mode detection of epinephrine and degradation of phenolic pollutants

  • 期刊论文

  • 发表日期：

    2025年3月28日

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  • Huizhu Fan,
  • Luxi Huang,
  • Yin Dai,
  • Hongsong Zhang,
  • Wanying Zhu,
  • Jun Zhang,
  • Junli Hong

摘要

Despite considerable interest has been shown in research on nanozymes in recent times, the creation of highly active bifunctional nanozymes remains a significant challenge. Inspired by the structural features of the active center of natural laccase, bifunctional copper-based nanozymes (Cu-MB) possessing both laccase-like activity and fluorescence properties using 2-methylimidazole (2-MI) and 2-aminoterephthalic acid (BDC-NH2) as ligands have been prepared. The Km of Cu-MB was 0.0898 mM, which was markedly lower than that of previously reported nanozymes and natural laccase. A dual-mode sensor for the detection of epinephrine (EP) was designed based on the high catalytic activity, excellent fluorescence properties, and stability of Cu-MB nanozymes. The limits of detection (LODs) of the colorimetric and fluorescence sensors for EP were 0.22 µg/mL and 0.11 µg/mL, respectively. The sensor system was verified to have good resistance to interferences and reliability by analyzing actual samples. Furthermore, the integration of a dual-reading hydrogel with a smartphone facilitates the portable, real-time, and rapid assessment of EP. More encouragingly, Cu-MB has excellent substrate versatility and can effectively degrade a diverse array of phenolic pollutants. The developed Cu-MB nanozymes provide a promising approach for the degradation and determination of phenolic compounds. Clinical trial number: not applicable.

Graphical abstract