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<p> A&#xa0;novel dendritic DNA-quantum dot (QD) electrochemiluminescence (ECL) probe was developed&#xa0;and an ECL biosensor constructed&#xa0;for sensitive detection of Hg<sup>2+</sup> in water samples by combining with enzyme-assisted multiple cycle amplification strategy. Firstly, the Y-shaped structure was formed based on the Hg<sup>2+</sup>-induced enzymatic cycle amplification technique, which improved the cutting efficiency and realized the double-amplified DNA product. Moreover, a unique dendritic DNA nanostructure loading numerous QDs was constructed, which can greatly amplify the&#xa0;ECL signal. After the dendritic DNA signal probe was connected to the CNT/gold nanocomposites/electrode by DNA products, the ECL biosensor was constructed for sensitive detection of Hg<sup>2+</sup>. The proposed dendritic DNA probe opens new ECL application of quantum dots. The smart design of Y-structure coupled with multiple amplification strategy greatly improves detection accuracy and sensitivity; thus, the biosensor not only can detect Hg<sup>2+</sup> in water samples, but also has a good application prospect for other targets in environmental analysis.</p> Graphical Abstract <p></p>

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Dendritic DNA/quantum dot nanostructure-based electrochemiluminescence biosensor for sensitive assay of Hg2+

  • 期刊论文

  • 发表日期：

    2025年2月21日

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  • Chuanbin Fan,
  • JunJun Ge,
  • Zhipeng Liang,
  • Guanghui Tian,
  • Ziao Zong,
  • Feng Guo,
  • Guifen Jie

摘要

A novel dendritic DNA-quantum dot (QD) electrochemiluminescence (ECL) probe was developed and an ECL biosensor constructed for sensitive detection of Hg2+ in water samples by combining with enzyme-assisted multiple cycle amplification strategy. Firstly, the Y-shaped structure was formed based on the Hg2+-induced enzymatic cycle amplification technique, which improved the cutting efficiency and realized the double-amplified DNA product. Moreover, a unique dendritic DNA nanostructure loading numerous QDs was constructed, which can greatly amplify the ECL signal. After the dendritic DNA signal probe was connected to the CNT/gold nanocomposites/electrode by DNA products, the ECL biosensor was constructed for sensitive detection of Hg2+. The proposed dendritic DNA probe opens new ECL application of quantum dots. The smart design of Y-structure coupled with multiple amplification strategy greatly improves detection accuracy and sensitivity; thus, the biosensor not only can detect Hg2+ in water samples, but also has a good application prospect for other targets in environmental analysis.

Graphical Abstract