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<p>A&#xa0;novel strategy is presented&#xa0;based on integration of nanostructures and molecular amplification was developed for detection of Let-7a miRNA as the carcinogenic cancer marker. Hybridization chain reaction (HCR) was triggered through the presence of Let-7a as the initiator target. This reaction resulted to the formation of AuNPs@CdS QDs assembly due to thiol bonding and following FRET occurred between free AuNPs and CdS QDs. Characterization and morphology of AuNPs@CdS QDs showed in situ accumulation of CdS QDs and afterward optical changes in fluorescence emission. The quenching of fluorescence emission was observed which corresponded&#xa0; to Let-7a concentration in the linear range 10–45 pM with LOD of 2 pM. The sensitivity and selectivity of the performed study confirmed the efficiency of the proposed strategy. Additionally, it demonstrated a high level of specificity and detection performance in real-sample analysis.</p> Graphical Abstract <p></p>

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Fluorescence-based detection of Let-7a miRNA through HCR-based approach upon the in situ interaction of AuNPs@CdS QDs and FRET mechanism

  • 期刊论文

  • 发表日期：

    2025年2月12日

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  • Farid Hosseini,
  • Mehdi Dadmehr,
  • Morteza Hosseini

摘要

A novel strategy is presented based on integration of nanostructures and molecular amplification was developed for detection of Let-7a miRNA as the carcinogenic cancer marker. Hybridization chain reaction (HCR) was triggered through the presence of Let-7a as the initiator target. This reaction resulted to the formation of AuNPs@CdS QDs assembly due to thiol bonding and following FRET occurred between free AuNPs and CdS QDs. Characterization and morphology of AuNPs@CdS QDs showed in situ accumulation of CdS QDs and afterward optical changes in fluorescence emission. The quenching of fluorescence emission was observed which corresponded  to Let-7a concentration in the linear range 10–45 pM with LOD of 2 pM. The sensitivity and selectivity of the performed study confirmed the efficiency of the proposed strategy. Additionally, it demonstrated a high level of specificity and detection performance in real-sample analysis.

Graphical Abstract