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<p>Ternary heterojunction Bi<sub>2</sub>S<sub>3</sub>/MoS<sub>2</sub>/Bi<sub>2</sub>MoO<sub>6</sub> was designed as a signal probe to develop a dual signal amplification strategy empowered electrochemical biosensor for sensitive miRNA-21 detection by combining with catalytic hairpin assembly (CHA). The combination of the Bi<sub>2</sub>S<sub>3</sub>/MoS<sub>2</sub>/Bi<sub>2</sub>MoO<sub>6</sub> heterojunction as a tracer indication probe and the CHA amplification strategy not only took fully use of the highly dense nanowire interwoven structure and superior active region of the probe, but also endowed the ability to improve the molecular hybridization efficiency by collision, which significantly avoided the cumbersome chain design and greatly simplified the step-by-step construction of the electrode surface. Hairpin H1 was first added dropwise to the gold nanoparticle-decorated electrode surface, and then opened by the introduced miRNA-21 to initiate the specific hybridization. Once the H2-MB/Bi<sub>2</sub>S<sub>3</sub>/MoS<sub>2</sub>/Bi<sub>2</sub>MoO<sub>6</sub> heterojunction was added, more sensitive and rapid detection of miRNA-21 would be achieved through the introduction of methylene blue since the cocatalysis of hairpin assembly and ternary heterojunction Bi<sub>2</sub>S<sub>3</sub>@MoS<sub>2</sub>@Bi<sub>2</sub>MoO<sub>6</sub>. Finally, the constructed biosensor demonstrated effective performance in the detection of miRNA-21 within the linear range of 1 fM to 100&#xa0;pM with a detection limit of 0.31 fM. The satisfactory analysis of human blood samples further validated the preferable reliability and practicability of this new strategy for the ultrasensitive detection of miRNA.</p> Graphical Abstract <p></p>

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Cocatalysis of catalytic hairpin assembly and ternary heterojunction Bi2S3@MoS2@Bi2MoO6 promotes ultra-sensitive electrochemical of detection MiRNA-21

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Ruizhuo Ouyang,
  • Yuanhui Ma,
  • Lan Jiang,
  • Xiaoqing Zhang,
  • Xi Liu,
  • Ying Wang,
  • Ying Huang,
  • Yuefeng Zhao,
  • Yuqin Jiang,
  • Yuqing Miao,
  • Baolin Liu

摘要

Ternary heterojunction Bi2S3/MoS2/Bi2MoO6 was designed as a signal probe to develop a dual signal amplification strategy empowered electrochemical biosensor for sensitive miRNA-21 detection by combining with catalytic hairpin assembly (CHA). The combination of the Bi2S3/MoS2/Bi2MoO6 heterojunction as a tracer indication probe and the CHA amplification strategy not only took fully use of the highly dense nanowire interwoven structure and superior active region of the probe, but also endowed the ability to improve the molecular hybridization efficiency by collision, which significantly avoided the cumbersome chain design and greatly simplified the step-by-step construction of the electrode surface. Hairpin H1 was first added dropwise to the gold nanoparticle-decorated electrode surface, and then opened by the introduced miRNA-21 to initiate the specific hybridization. Once the H2-MB/Bi2S3/MoS2/Bi2MoO6 heterojunction was added, more sensitive and rapid detection of miRNA-21 would be achieved through the introduction of methylene blue since the cocatalysis of hairpin assembly and ternary heterojunction Bi2S3@MoS2@Bi2MoO6. Finally, the constructed biosensor demonstrated effective performance in the detection of miRNA-21 within the linear range of 1 fM to 100 pM with a detection limit of 0.31 fM. The satisfactory analysis of human blood samples further validated the preferable reliability and practicability of this new strategy for the ultrasensitive detection of miRNA.

Graphical Abstract