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<p>A&#xa0;biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS<sub>2</sub> nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover,&#xa0;multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a <b>G-</b>quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron. In the absence of MiR-31, the tetrahedron DNAzyme probe formed after the addition of hemin can mediate the deposition of insoluble on MoS<sub>2</sub>/AAO, which not only enables the color change of the membrane but also gates the transport of K<sub>3</sub>[Fe(CN)<sub>6</sub>] across the nanochannels. Therefore, the detection of MiR-31 is realized by both visual observation of the brown color and measuring the electrochemical redox current of [Fe(CN)<sub>6</sub>]<sup>3<b>−</b></sup>. Using this biosensor, a detection limit as low as 0.06&#xa0;fM is achieved. The&#xa0;dual-mode detection method also exhibits good specificity, reproducibility, and stability, demonstrating potential application in the diagnosis of oral squamous cell carcinoma and other related biological purposes.</p> Graphical Abstract <p></p>

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A dual-mode biosensor for microRNA detection based on DNA tetrahedron-gated nanochannels

  • 期刊论文

  • 发表日期：

    2025年1月18日

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  • Yuan Su,
  • Ziyu Zhang,
  • Hongfang Zhang

摘要

A biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS2 nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover, multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a G-quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron. In the absence of MiR-31, the tetrahedron DNAzyme probe formed after the addition of hemin can mediate the deposition of insoluble on MoS2/AAO, which not only enables the color change of the membrane but also gates the transport of K3[Fe(CN)6] across the nanochannels. Therefore, the detection of MiR-31 is realized by both visual observation of the brown color and measuring the electrochemical redox current of [Fe(CN)6]3−. Using this biosensor, a detection limit as low as 0.06 fM is achieved. The dual-mode detection method also exhibits good specificity, reproducibility, and stability, demonstrating potential application in the diagnosis of oral squamous cell carcinoma and other related biological purposes.

Graphical Abstract