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<p> A&#xa0;fluorescent aptasensor was developed&#xa0;based on target-induced hairpin conformation switch coupled with nicking enzyme-assisted signal amplification (NESA) to detect the oligomeric form of ß-amyolid&#xa0;peptide (AβO) in cerebrospinal fluid. The hairpin DNA probe (HP) was specifically designed to recognize AβO. When AβO is present in the sensing system, it induces an HP conformational switch and triggers the NESA reaction. After evaluating the parameters of the aptasensor system, we selected Hairpin10, which has a 10-nucleotide extended sequence, as the hairpin sequence that interacts with AβO. The quantitative linear range of the proposed aptasensor is from&#xa0;11.3 to 113 ng mL<sup>−1</sup> in artificial cerebrospinal fluid (aCSF), and the detection limit was 7.29 ng mL<sup>−1</sup>. The present work realized the assay of AβO in aCSF with satisfactory quantitative results.</p> Graphical abstract <p></p>

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Fluorescent aptasensor based on target-induced hairpin conformation switch coupled with nicking enzyme-assisted signal amplification for detection of beta-amyloid oligomers in cerebrospinal fluid

  • 期刊论文

  • 发表日期：

    2025年1月13日

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  • Wan-Chen Chuang,
  • Chun-Hsien Chen,
  • Tsai-Hui Duh,
  • Yen-Ling Chen

摘要

A fluorescent aptasensor was developed based on target-induced hairpin conformation switch coupled with nicking enzyme-assisted signal amplification (NESA) to detect the oligomeric form of ß-amyolid peptide (AβO) in cerebrospinal fluid. The hairpin DNA probe (HP) was specifically designed to recognize AβO. When AβO is present in the sensing system, it induces an HP conformational switch and triggers the NESA reaction. After evaluating the parameters of the aptasensor system, we selected Hairpin10, which has a 10-nucleotide extended sequence, as the hairpin sequence that interacts with AβO. The quantitative linear range of the proposed aptasensor is from 11.3 to 113 ng mL−1 in artificial cerebrospinal fluid (aCSF), and the detection limit was 7.29 ng mL−1. The present work realized the assay of AβO in aCSF with satisfactory quantitative results.

Graphical abstract