Scholarlink

<p>Unique rhenium disulfide/reduced graphene oxide (ReS<sub>2</sub>/rGO) nanoframeworks were synthesized with a hierarchical layered and porous structure for the ultrasensitive electrochemical detection of microRNA-21 (miRNA-21) by empowering multi-signal amplification strategy of catalytic hairpin self-assembly-hybridization chain reaction (CHA-HCR). The layered and porous nanostructures endowed ReS<sub>2</sub>/rGO with a larger specific surface area and more active sites through connecting vertical ReS<sub>2</sub> with rGO which was preferable for promoting the electron transfer over electrode surface because of a conductive network. This nanoframework facilitated the loading of adequate gold nanoparticles to fix the capture probe via Au–S bond. In the presence of the target miRNA-21, the CHA-HCR double amplification reaction could be triggered to generate a long double strand with methylene blue (MB) embedded inside. The electrochemical sensing platform was thus empowered by the unique ReS<sub>2</sub>/rGO nanoframeworks to detect miRNA-21 in the range 1 fM ~ 100&#xa0;pM with the remarkably enhanced sensitivity through detecting the significantly amplified signal from the REDOX reaction of MB inside the long chain. The verification of the miRNA-21 detection in real blood samples further proved the great potential of this new method with the limit of detection reduced down to 0.057 fM and opens a new window for ReS<sub>2</sub> in developing sensitive biosensors for early clinical cancer diagnosis.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Vertical growth of rhenium disulfide on rGO empowers multi-signal amplification for ultrasensitive MiRNA-21 detection

  • 期刊论文

  • 发表日期：

    2025年2月19日

查看全文
  • Ruizhuo Ouyang,
  • Ying Huang,
  • Haohao Chen,
  • Yuanhui Ma,
  • Tai Ye,
  • Xi Liu,
  • Ying Wang,
  • Yuefeng Zhao,
  • Yuqin Jiang,
  • Yuqing Miao,
  • Baolin Liu

摘要

Unique rhenium disulfide/reduced graphene oxide (ReS2/rGO) nanoframeworks were synthesized with a hierarchical layered and porous structure for the ultrasensitive electrochemical detection of microRNA-21 (miRNA-21) by empowering multi-signal amplification strategy of catalytic hairpin self-assembly-hybridization chain reaction (CHA-HCR). The layered and porous nanostructures endowed ReS2/rGO with a larger specific surface area and more active sites through connecting vertical ReS2 with rGO which was preferable for promoting the electron transfer over electrode surface because of a conductive network. This nanoframework facilitated the loading of adequate gold nanoparticles to fix the capture probe via Au–S bond. In the presence of the target miRNA-21, the CHA-HCR double amplification reaction could be triggered to generate a long double strand with methylene blue (MB) embedded inside. The electrochemical sensing platform was thus empowered by the unique ReS2/rGO nanoframeworks to detect miRNA-21 in the range 1 fM ~ 100 pM with the remarkably enhanced sensitivity through detecting the significantly amplified signal from the REDOX reaction of MB inside the long chain. The verification of the miRNA-21 detection in real blood samples further proved the great potential of this new method with the limit of detection reduced down to 0.057 fM and opens a new window for ReS2 in developing sensitive biosensors for early clinical cancer diagnosis.

Graphical Abstract