<p>This study was conducted to evaluate clinical utility of serum neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) as novel diagnostic biomarkers in chronic kidney disease (CKD) in cats. A total number of 15 cats admitted to the Teaching Small Animal Hospital, Faculty of Veterinary Medicine, Cairo University, were included in this study. Cats were divided into two groups: six clinically healthy cats as the control group and nine diseased cats. All cats were subjected to complete clinical evaluation and ultrasonographic and hematobiochemical examination. Based on clinical and laboratory findings, nine cats were diagnosed with chronic kidney disease. The level of KIM-1 was measured using commercially available Cat KIM-1 ELISA kit and NGAL using Cat NGAL ELISA kit. Clinically, CKD was manifested by anorexia, polyuria, and polydipsia, with non-specific systemic signs including vomiting, melena, and depression. Ultrasonography revealed increased cortical and medullary echogenicity with reduced corticomedullary differentiation was observed in five cases. Multiple cavitary lesions were observed in renal parenchyma as oval lesions with hyperechoic thin wall filled with anechoic fluid causing distortion of renal tissue recorded in four cases. Hematological examination shows a significant decrease of red blood cell count, packed cell volume, and hemoglobin concentration with lymphocytosis. Serum biochemical analysis showed a significant increase of creatinine concentration, blood urea nitrogen, potassium, and phosphorus. Serum KIM-1 and NGAL levels significantly increased in CKD group. Serum NGAL and KIM-1 showed potential as diagnostic for chronic kidney disease in cats.</p>