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<p>The current study sought to elucidate and complete the development of iron-deficiency anemia. IDA animal model by investigating various factors, including hematology and histological analysis, using the AIN-93G, an iron-deficient diet. In this study, we used 30 Wistar rats with a body weight of 200–250&#xa0;g and a normal Hb range of &gt; 18.0&#xa0;g/dL. The study design consisted of six groups, each comprising five rats. The control groups were fed a standard diet for 60&#xa0;days, while the test groups were fed a low-iron diet (AIN-93G). To conduct the hematological and immunoassay studies, blood was collected from the retro-orbital region of both the control and test groups at different time points. The hemoglobin levels in the test groups were significantly lower than those in the control groups. The data was analyzed statistically using paired t-tests to differentiate between the control and test groups. <i>p</i> &lt; 0.05&#xa0;was considered to be the level of significance. After the end of the study, one rat from each group was sacrificed, and organs (brain, heart, liver, kidney, spleen, and lungs) were carefully dissected for histological analysis. This study successfully established the IDA animal model by modifying the standard diet to include iron-free corn oil and an iron-deficient mineral mix for 60&#xa0;days.</p>

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Development and standardization of iron deficient anemic model in Wistar albino rats using laboratory prepared iron deficient diet

  • 期刊论文

  • 发表日期：

    2026年1月24日

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  • Kuldeep Singh,
  • Dimple Sethi Chopra,
  • Dhandeep Singh,
  • Nirmal Singh

摘要

The current study sought to elucidate and complete the development of iron-deficiency anemia. IDA animal model by investigating various factors, including hematology and histological analysis, using the AIN-93G, an iron-deficient diet. In this study, we used 30 Wistar rats with a body weight of 200–250 g and a normal Hb range of > 18.0 g/dL. The study design consisted of six groups, each comprising five rats. The control groups were fed a standard diet for 60 days, while the test groups were fed a low-iron diet (AIN-93G). To conduct the hematological and immunoassay studies, blood was collected from the retro-orbital region of both the control and test groups at different time points. The hemoglobin levels in the test groups were significantly lower than those in the control groups. The data was analyzed statistically using paired t-tests to differentiate between the control and test groups. p < 0.05 was considered to be the level of significance. After the end of the study, one rat from each group was sacrificed, and organs (brain, heart, liver, kidney, spleen, and lungs) were carefully dissected for histological analysis. This study successfully established the IDA animal model by modifying the standard diet to include iron-free corn oil and an iron-deficient mineral mix for 60 days.