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<p>Dielectrophoresis (DEP) is widely used for label-free cell separation in microfluidic systems; however, conventional planar geometries often require particle pre-focusing and relatively high operating voltages. In this study, the continuous separation of red blood cells (RBCs) and MDA-MB-231 breast cancer cells is numerically investigated in a cylindrical microchannel with a circular cross-section. COMSOL Multiphysics simulations are performed to evaluate the effects of channel width, channel length, flow rate, and applied voltage on the minimum separation voltage. The results show that channel width strongly influences the required voltage. Reducing the channel width from 450&#xa0;μm to 100&#xa0;μm decreases the minimum separation voltage from 18.5&#xa0;V to 3.25&#xa0;V for RBCs and from 13.5&#xa0;V to 2.25&#xa0;V for cancer cells, corresponding to a reduction of more than 80%. Increasing channel length reduces the required voltage due to longer residence time, whereas increasing flow rate has the opposite effect. Under the selected conditions (width = 100&#xa0;μm, length = 10&#xa0;mm, flow rate = 10 µL/min), numerical simulations predict complete trajectory separation of the two cell types at a minimum applied voltage of 3.25&#xa0;V. The proposed cylindrical configuration provides a simplified geometry for continuous DEP-based cell separation without upstream hydrodynamic focusing, although experimental validation is required to confirm the predicted separation performance.</p>

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Design and simulation of a cylindrical DEP microchannel for continuous separation of circulating tumor cells and red blood cells

  • 期刊论文

  • 发表日期：

    2026年7月27日

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  • Hamid Jannat Rostami,
  • Abas Ramiar,
  • Reza Derakhshan

摘要

Dielectrophoresis (DEP) is widely used for label-free cell separation in microfluidic systems; however, conventional planar geometries often require particle pre-focusing and relatively high operating voltages. In this study, the continuous separation of red blood cells (RBCs) and MDA-MB-231 breast cancer cells is numerically investigated in a cylindrical microchannel with a circular cross-section. COMSOL Multiphysics simulations are performed to evaluate the effects of channel width, channel length, flow rate, and applied voltage on the minimum separation voltage. The results show that channel width strongly influences the required voltage. Reducing the channel width from 450 μm to 100 μm decreases the minimum separation voltage from 18.5 V to 3.25 V for RBCs and from 13.5 V to 2.25 V for cancer cells, corresponding to a reduction of more than 80%. Increasing channel length reduces the required voltage due to longer residence time, whereas increasing flow rate has the opposite effect. Under the selected conditions (width = 100 μm, length = 10 mm, flow rate = 10 µL/min), numerical simulations predict complete trajectory separation of the two cell types at a minimum applied voltage of 3.25 V. The proposed cylindrical configuration provides a simplified geometry for continuous DEP-based cell separation without upstream hydrodynamic focusing, although experimental validation is required to confirm the predicted separation performance.