<p>In this paper, we establish a class of Stein-Weiss type inequality with partial variable weight functions on the upper half space using a weighted Hardy type inequality. Overcoming the impact of weighted functions, the existence of extremal functions is proved via the concentration compactness principle, whereas Riesz rearrangement inequality is not available. Moreover, the cylindrical symmetry with respect to the <i>t</i>-axis and the explicit forms on the boundary of all nonnegative extremal functions are discussed via the method of moving planes and method of moving spheres, as well as, regularity results are obtained by the regularity lift lemma and bootstrap technique. As applications, we obtain some weighted Sobolev inequalities with partial variable weight function for Laplacian and fractional Laplacian.</p>