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<Emphasis Type="BoldItalic">Key message</Emphasis> <p><b>The developmental stage of cotyledonary explants influences morphogenesis in</b> <Emphasis Type="BoldItalic">Delonix regia</Emphasis><b>, with immature and seedling-derived explants being more responsive to in vitro regeneration.</b></p> Abstract <p>The developmental stage of the explants plays a crucial role in the expression of morphogenesis. This study aimed to evaluate the morphophysiological behavior of cotyledonary explants at different developmental stages during somatic embryogenesis induction of <i>Delonix regia</i> (Bojer ex Hook.) Raf. cotyledons from seedlings (SC), immature (IC), and mature (MC) zygotic embryos were inoculated in MS culture medium with 1.0 mg L<sup>− 1</sup> of dichlorophenoxyacetic acid (2,4-D) and&#xa0;0.125 mg L<sup>− 1</sup> of 6-benzyladenine (BA). The experiment was conducted in a growth room at 25 ± 1&#xa0;°C, in the dark, for 30 days. The IC and SC presented calluses on their surface and gained fresh mass. These calluses showed cell clusters with meristematic-like cytological features, confirming the morphogenetic potential of the calluses. In contrast, MC did not present morphogenetic response or cytological changes. Unlike the IC and SC storage contents, the MC protein, lipid, and carbohydrate contents did not change significantly throughout the in vitro culture. Interestingly, explants that showed morphogenetic responses (IC and SC) had significantly higher indole-3-acetic acid, proline, and putrescine content than MC. The results obtained demonstrate the influence of the development stage of cotyledonary explants for the induction of morphogenetic pathways and contribute to a better understanding of the in vitro behavior of cotyledonary explants for the establishment of regeneration systems of <i>D. regia</i> and other woody species.</p>

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Developmental stage dictates the morphogenetic competence of royal poinciana [Delonix regia (Bojer ex Hook.) Raf. cotyledons

  • 期刊论文

  • 发表日期：

    2026年2月13日

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  • Andrey de Oliveira Costa,
  • Lázara Aline Simões Silva,
  • Otalício Damásio da Costa Junior,
  • Thamires Fernanda Gomes,
  • Josimar dos Santos Ladeira,
  • Mariana Machado,
  • Leandro Silva de Oliveira,
  • Diego Ismael Rocha

摘要

Key message

The developmental stage of cotyledonary explants influences morphogenesis in Delonix regia, with immature and seedling-derived explants being more responsive to in vitro regeneration.

Abstract

The developmental stage of the explants plays a crucial role in the expression of morphogenesis. This study aimed to evaluate the morphophysiological behavior of cotyledonary explants at different developmental stages during somatic embryogenesis induction of Delonix regia (Bojer ex Hook.) Raf. cotyledons from seedlings (SC), immature (IC), and mature (MC) zygotic embryos were inoculated in MS culture medium with 1.0 mg L− 1 of dichlorophenoxyacetic acid (2,4-D) and 0.125 mg L− 1 of 6-benzyladenine (BA). The experiment was conducted in a growth room at 25 ± 1 °C, in the dark, for 30 days. The IC and SC presented calluses on their surface and gained fresh mass. These calluses showed cell clusters with meristematic-like cytological features, confirming the morphogenetic potential of the calluses. In contrast, MC did not present morphogenetic response or cytological changes. Unlike the IC and SC storage contents, the MC protein, lipid, and carbohydrate contents did not change significantly throughout the in vitro culture. Interestingly, explants that showed morphogenetic responses (IC and SC) had significantly higher indole-3-acetic acid, proline, and putrescine content than MC. The results obtained demonstrate the influence of the development stage of cotyledonary explants for the induction of morphogenetic pathways and contribute to a better understanding of the in vitro behavior of cotyledonary explants for the establishment of regeneration systems of D. regia and other woody species.