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<p>Gibberellins (GAs) are key plant growth regulators, but their regulatory mechanisms in woody plants remain to be explored. In this study, <i>Populus davidiana</i> × <i>P. bolleana</i> (Shanxin poplar) plants were treated with GA<sub>3</sub> for different times. The results revealed that exogenous GA<sub>3</sub> increased the fresh weight, stem internode length, plant height, stem diameter, chlorophyll content, and the width and length of leaves. Xylogenesis was enhanced under GA<sub>3</sub> treatment, as evidenced by the staining of transverse stem sections with toluidine blue, phloroglucinol-HCl, and calcofluor white. In addition, the total contents of endogenous GA, auxin, and cytokinin were increased. Furthermore, RNA-seq analysis indicated that 112, 2473, and 3773 genes were differentially expressed under GA<sub>3</sub> treatment for 0.5&#xa0;h, 48&#xa0;h, and 7 d. The differentially expressed genes (DEGs) were mainly related to plant hormone signal transduction, phenylpropanoid biosynthesis, diterpenoid biosynthesis, and photosynthesis processes. Further analysis suggested that GA<sub>3</sub> treatment enhanced plant biomass by regulating the expression of DEGs involved in cell elongation, xylem formation, and stress tolerance. Furthermore, our research has demonstrated that the overexpression of the DEGs MYB52 and bHLH71 can significantly enhance the salt tolerance of poplar plants by improving their capacity to scavenge reactive oxygen species (ROS). These findings provide insight into the regulatory mechanisms of GA<sub>3</sub> and identify the key genes associated with high salt tolerance in Shanxin poplar.</p>

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Regulatory mechanisms of exogenous GA3 application and identification of key genes related to salt tolerance in Populus davidiana × P. bolleana

  • 期刊论文

  • 发表日期：

    2025年6月16日

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  • Fangyu Lin,
  • Qi Jiang,
  • Hu Sun,
  • Yucheng Wang,
  • Huiyan Guo

摘要

Gibberellins (GAs) are key plant growth regulators, but their regulatory mechanisms in woody plants remain to be explored. In this study, Populus davidiana × P. bolleana (Shanxin poplar) plants were treated with GA3 for different times. The results revealed that exogenous GA3 increased the fresh weight, stem internode length, plant height, stem diameter, chlorophyll content, and the width and length of leaves. Xylogenesis was enhanced under GA3 treatment, as evidenced by the staining of transverse stem sections with toluidine blue, phloroglucinol-HCl, and calcofluor white. In addition, the total contents of endogenous GA, auxin, and cytokinin were increased. Furthermore, RNA-seq analysis indicated that 112, 2473, and 3773 genes were differentially expressed under GA3 treatment for 0.5 h, 48 h, and 7 d. The differentially expressed genes (DEGs) were mainly related to plant hormone signal transduction, phenylpropanoid biosynthesis, diterpenoid biosynthesis, and photosynthesis processes. Further analysis suggested that GA3 treatment enhanced plant biomass by regulating the expression of DEGs involved in cell elongation, xylem formation, and stress tolerance. Furthermore, our research has demonstrated that the overexpression of the DEGs MYB52 and bHLH71 can significantly enhance the salt tolerance of poplar plants by improving their capacity to scavenge reactive oxygen species (ROS). These findings provide insight into the regulatory mechanisms of GA3 and identify the key genes associated with high salt tolerance in Shanxin poplar.