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Key message <p><b>Transient overexpression of </b><Emphasis Type="BoldItalic">PeSUS3</Emphasis> <b>using a</b> <Emphasis Type="BoldItalic">BaMV</Emphasis> <b>vector in</b><Emphasis Type="BoldItalic"> D. brandisii</Emphasis> <b>‘Manxie Tianzhu’ enhanced tillering bud germination and growth, suggesting a crucial role for sucrose metabolism in bamboo development.</b></p> Abstract <p>Bamboo shoots are highly sought after in China for their nutritional value. <i>Dendrocalamus brandisii</i> ‘Manxie Tianzhu’ (Manxie Sweet Dragon Bamboo) faces supply challenges due to limited distribution, necessitating faster growing, higher yielding varieties. The sucrose synthase gene, particularly <i>PeSUS3</i>, is vital for sucrose metabolism, nutrient transport, and regulating dormancy release and bud germination, yet its role in bamboo shoot production is not well understood. This study used a bamboo mosaic virus vector (BaMV) to overexpress <i>PeSUS3</i> in <i>D. brandisii</i> ‘Manxie Tianzhu’ seedlings. <i>PeSUS3</i> was cloned into the BaMV-P19-EGFP-2 vector, introduced into <i>Agrobacterium tumefaciens</i>, and used to infect tobacco leaves. The resulting virus was mechanically inoculated into the bamboo seedlings. Analysis revealed that <i>PeSUS3</i> overexpression increased the number of tiller buds and accelerated germination by 1–2&#xa0;days compared to controls. Despite the transient overexpression of <i>PeSUS3</i>, the increased sucrose content in the overexpressing plants suggests a role for sucrose metabolism in these processes. These findings emphasize the importance of the transient overexpression of <i>PeSUS3</i> in promoting tillering bud germination and growth in <i>D. brandisii</i> ‘Manxie Tianzhu’, providing a foundation for future research on its function and mechanisms.</p>

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Moso bamboo sucrose synthase gene SUS3 promotes earlier germination of tiller buds in Dendrocalamus brandisii ‘Manxie Tianzhu’

  • 期刊论文

  • 发表日期：

    2025年2月25日

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  • Weijie Sun,
  • Muthusamy Ramakrishnan,
  • Long-Hai Zou,
  • Kai-Li Wang,
  • Xiaohong Zhou,
  • Mingbing Zhou

摘要

Key message

Transient overexpression of PeSUS3 using a BaMV vector in D. brandisii ‘Manxie Tianzhu’ enhanced tillering bud germination and growth, suggesting a crucial role for sucrose metabolism in bamboo development.

Abstract

Bamboo shoots are highly sought after in China for their nutritional value. Dendrocalamus brandisii ‘Manxie Tianzhu’ (Manxie Sweet Dragon Bamboo) faces supply challenges due to limited distribution, necessitating faster growing, higher yielding varieties. The sucrose synthase gene, particularly PeSUS3, is vital for sucrose metabolism, nutrient transport, and regulating dormancy release and bud germination, yet its role in bamboo shoot production is not well understood. This study used a bamboo mosaic virus vector (BaMV) to overexpress PeSUS3 in D. brandisii ‘Manxie Tianzhu’ seedlings. PeSUS3 was cloned into the BaMV-P19-EGFP-2 vector, introduced into Agrobacterium tumefaciens, and used to infect tobacco leaves. The resulting virus was mechanically inoculated into the bamboo seedlings. Analysis revealed that PeSUS3 overexpression increased the number of tiller buds and accelerated germination by 1–2 days compared to controls. Despite the transient overexpression of PeSUS3, the increased sucrose content in the overexpressing plants suggests a role for sucrose metabolism in these processes. These findings emphasize the importance of the transient overexpression of PeSUS3 in promoting tillering bud germination and growth in D. brandisii ‘Manxie Tianzhu’, providing a foundation for future research on its function and mechanisms.