Background <p>The management of Henle’s Trunk is a critical step in right hemicolectomy. However, there are few reports detailing standardized methods for safe vascular ligation. In this study, we will introduce a 4-step method for safe handling of Henle’s Trunk. </p> Methods <p>We retrospectively analyzed laparoscopic videos of the right hemicolectomy for Henle’s Trunk, and classified the key procedure steps into the four steps. Operative time spent on the management of Henle’s Trunk, intraoperative bleeding and operative outcomes were analyzed by reviewing the surgical videos and clinical information. </p> Results <p>A total of 25 patients completed the management of Henle’s Trunk successfully, and the average operative time for Henle’s trunk was 25.13&#xa0;min. The median intraoperative blood loss with interquartile ranges was 3(2)&#xa0;ml and no patient had uncontrollable bleeding or accidental injury during the procedure. Learning curve analysis suggested that 12 cases could be familiarized with the 4-step procedure.</p> Conclusion <p>The 4-step procedure is safe and effective for management of Henle’s Trunk branches in right hemicolectomy, and the method has a short learning curve, which can be appropriately promoted.</p> Graphical abstract <p></p>