We analyze the hit-and-run algorithm for sampling uniformly from an isotropic convex body K in n dimensions. We show that the algorithm mixes in \(\epsilon \) -total variation error with \(O_\epsilon (n^2/ \psi _n^2)\) steps, where \(\psi _n\) is the smallest isoperimetric constant for any isotropic logconcave distribution, also known as the Kannan-Lovasz-Simonovits (KLS) constant [19]. Our bound improves upon previous bounds of the form \(O_\epsilon (n^2 R^2/r^2)\) , which depend on the ratio R/r of the radii of the circumscribed and inscribed balls of K, gaining a factor of n in the case of isotropic convex bodies. Consequently, our result gives a mixing time estimate for the hit-and-run which matches the state-of-the-art bounds for the ball walk. Our main proof technique is based on an annealing of localization schemes introduced in Chen and Eldan [7], which allows us to reduce the problem to the analysis of the mixing time on truncated Gaussian distributions.