Scholarlink

<p><i>Rhodosporidium toruloides</i> is widely recognized for its capacity to accumulate high-value carotenoids with applications across food, pharmaceutical, and cosmetic sectors. Despite this potential, the recovery of microbial carotenoids remains constrained by conventional extraction protocols that rely on several steps and the utilization of considerable volumes of chemicals, which increase chemical demand, processing time, and energy consumption. In this study, a one-pot sonication method was developed in which cell disruption and carotenoid extraction occur simultaneously. A Box–Behnken design was used to optimize temperature, extraction time, and broth-to-methanol ratio, showing 60&#xa0;°C, 20&#xa0;min, and a 1:1 broth-to-solvent ratio as the optimal conditions. Under these conditions, the method achieved an extraction efficiency of 120.2% with 2.46 ± 0.12&#xa0;mg/L, representing a 1.2-fold improvement over the conventional acid–acetone protocol. The one-pot method reduced solvent consumption by 91.4% and decreased total energy use from 6750.27&#xa0;kJ to 4242&#xa0;kJ (a 37% reduction). Furthermore, scale-up to 500 mL yielded 2.76 ± 0.18&#xa0;mg/L of carotenoids, confirming robustness and reproducibility. In general, the method provides an energy-efficient and sustainable alternative for microbial carotenoid extraction.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated one-pot ultrasound-assisted method for carotenoid extraction from Rhodosporidium toruloides

  • 期刊论文

  • 发表日期：

    2026年3月4日

查看全文
  • Lachi Wankhede,
  • Gaurav Bhardwaj,
  • Carlos Saul Osorio-González,
  • Satinder Kaur Brar

摘要

Rhodosporidium toruloides is widely recognized for its capacity to accumulate high-value carotenoids with applications across food, pharmaceutical, and cosmetic sectors. Despite this potential, the recovery of microbial carotenoids remains constrained by conventional extraction protocols that rely on several steps and the utilization of considerable volumes of chemicals, which increase chemical demand, processing time, and energy consumption. In this study, a one-pot sonication method was developed in which cell disruption and carotenoid extraction occur simultaneously. A Box–Behnken design was used to optimize temperature, extraction time, and broth-to-methanol ratio, showing 60 °C, 20 min, and a 1:1 broth-to-solvent ratio as the optimal conditions. Under these conditions, the method achieved an extraction efficiency of 120.2% with 2.46 ± 0.12 mg/L, representing a 1.2-fold improvement over the conventional acid–acetone protocol. The one-pot method reduced solvent consumption by 91.4% and decreased total energy use from 6750.27 kJ to 4242 kJ (a 37% reduction). Furthermore, scale-up to 500 mL yielded 2.76 ± 0.18 mg/L of carotenoids, confirming robustness and reproducibility. In general, the method provides an energy-efficient and sustainable alternative for microbial carotenoid extraction.

Graphical Abstract