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<p>Lactate-, ethanol- and citrate-rich side streams are increasingly available from waste fermentation and organic-acid industries, yet their effects on carbon allocation in mixed-culture activated sludge remain insufficiently compared under identical conditions. Here, we operated three parallel sequencing batch reactors fed with lactate, ethanol, or citrate and quantified treatment performance together with chemical oxygen demand (COD) partitioning to intracellular polyhydroxyalkanoates (PHA) and extracellular polymeric substances (EPS). All reactors maintained stable removals of COD, total nitrogen, and total phosphorus above 80%. However, carbon allocation differed strongly. Biomass accumulated about 20 to 30&#xa0;mg/g volatile suspended solids, corresponding to COD to PHA recoveries of 60.7% for lactate, 37.9% for ethanol, and 39.6% for citrate. Lactate minimized the non-PHA COD fraction and produced polyhydroxybutyrate-valerate with the highest hydroxyvalerate share, whereas ethanol favored polyhydroxybutyrate rich PHA and diverted more carbon to EPS and respiration. The EPS matrix was protein-dominated and more humified under ethanol, moderately protein-rich under lactate, and more polysaccharide-biased with weaker humic-like signals under citrate. Community profiling supported these shifts, with lactate enriching storage-oriented guilds, ethanol enriching denitrifying and EPS-producing guilds, and citrate supporting an enhanced biological phosphorus removal like community. Together, these results show that substrate chemistry beyond conventional volatile fatty acids can be used to steer the balance between PHA storage and EPS formation while maintaining nutrient removal.</p>

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Bioprocess performance and COD partitioning for PHA/EPS production from activated sludge fed with lactate, ethanol and citrate

  • 期刊论文

  • 发表日期：

    2026年2月20日

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  • Teng Sun,
  • Quan Yuan,
  • Mengjia Xiao,
  • Xinyu Zhang,
  • Yingxue Sun,
  • Mingquan Huang,
  • Jihong Wu,
  • Xueting Li,
  • Liang Qian

摘要

Lactate-, ethanol- and citrate-rich side streams are increasingly available from waste fermentation and organic-acid industries, yet their effects on carbon allocation in mixed-culture activated sludge remain insufficiently compared under identical conditions. Here, we operated three parallel sequencing batch reactors fed with lactate, ethanol, or citrate and quantified treatment performance together with chemical oxygen demand (COD) partitioning to intracellular polyhydroxyalkanoates (PHA) and extracellular polymeric substances (EPS). All reactors maintained stable removals of COD, total nitrogen, and total phosphorus above 80%. However, carbon allocation differed strongly. Biomass accumulated about 20 to 30 mg/g volatile suspended solids, corresponding to COD to PHA recoveries of 60.7% for lactate, 37.9% for ethanol, and 39.6% for citrate. Lactate minimized the non-PHA COD fraction and produced polyhydroxybutyrate-valerate with the highest hydroxyvalerate share, whereas ethanol favored polyhydroxybutyrate rich PHA and diverted more carbon to EPS and respiration. The EPS matrix was protein-dominated and more humified under ethanol, moderately protein-rich under lactate, and more polysaccharide-biased with weaker humic-like signals under citrate. Community profiling supported these shifts, with lactate enriching storage-oriented guilds, ethanol enriching denitrifying and EPS-producing guilds, and citrate supporting an enhanced biological phosphorus removal like community. Together, these results show that substrate chemistry beyond conventional volatile fatty acids can be used to steer the balance between PHA storage and EPS formation while maintaining nutrient removal.