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<p>Volatile fatty acids (VFAs) derived from organic waste offer promising and cost-effective carbon sources for the production of microbial lipids. This study demonstrates the significant influence of nitrogen nutrition on cell proliferation and microbial lipid synthesis in <i>Yarrowia lipolytica</i> during high-concentration acid cultivation. Further investigations into nitrogen sources revealed that NH<sub>4</sub>Cl and urea are suitable options for cultivating <i>Y. lipolytica</i> to produce microbial lipids, resulting in lipid yields ranging from 2.00 to 2.50&#xa0;g/L. Moreover, pH fluctuations were found to be influenced by both the nitrogen source and acid utilisation, with pH adaptation helping alleviate acid inhibition caused by high-concentration VFAs. Under optimised cultivation conditions, the highest yield of microbial lipids reached 4.00&#xa0;g/L, accompanied by a dry cell weight of 9.91&#xa0;g/L and a microbial lipid content of 40.37%, consisting predominantly of C16 ~ 18 fatty acids. These findings highlight the central role of nitrogen metabolism and pH adaptation in enhancing VFA assimilation, offering guidance for cost-effective microbial lipid production from organic waste streams.</p>

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Regulatory effects of carbon and nitrogen nutrition on lipid accumulation by Yarrowia lipolytica cultivated with high-concentration volatile fatty acids

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月3日

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  • Xuemei Wang,
  • Gehang Yuan,
  • Meiyan Li,
  • Shushuang Sun,
  • Shikun Cheng,
  • Luiza C. Campos,
  • Zifu Li

摘要

Volatile fatty acids (VFAs) derived from organic waste offer promising and cost-effective carbon sources for the production of microbial lipids. This study demonstrates the significant influence of nitrogen nutrition on cell proliferation and microbial lipid synthesis in Yarrowia lipolytica during high-concentration acid cultivation. Further investigations into nitrogen sources revealed that NH4Cl and urea are suitable options for cultivating Y. lipolytica to produce microbial lipids, resulting in lipid yields ranging from 2.00 to 2.50 g/L. Moreover, pH fluctuations were found to be influenced by both the nitrogen source and acid utilisation, with pH adaptation helping alleviate acid inhibition caused by high-concentration VFAs. Under optimised cultivation conditions, the highest yield of microbial lipids reached 4.00 g/L, accompanied by a dry cell weight of 9.91 g/L and a microbial lipid content of 40.37%, consisting predominantly of C16 ~ 18 fatty acids. These findings highlight the central role of nitrogen metabolism and pH adaptation in enhancing VFA assimilation, offering guidance for cost-effective microbial lipid production from organic waste streams.