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<p><i>Yarrowia lipolytica</i> has been studied for poly (ethylene terephthalate) (PET) depolymerization, but the slow kinetics must be improved for large-scale applications. Here, dimethyl sulfoxide (DMSO) was added to a medium containing post-consumer PET (PC-PET) or the monomers terephthalic acid (TPA), bis(hydroxy-ethylene) terephthalate (BHET), and methyl-2-hydroxy ethylene terephthalate (MHET) to increase its solubility and improve depolymerization. The MIC test indicated 5% of DMSO as the maximum non-toxic concentration for <i>Y. lipolytica</i> cultivation. Cell viability on yeast nitrogen-based (YNB) medium was higher with MHET (94%). Cell growth in YNB medium and PC-PET was only detected with DMSO. When PC-PET was used as an additional carbon source, cell growth was 40% higher in the presence of DMSO (10.7&#xa0;g/L), exhibiting increased adhesion of cells to PET (20%). Also, the highest extracellular lipase activity (370&#xa0;U/L) was found with DMSO and PC-PET in flasks. In a bioreactor, higher cell growth (32.6&#xa0;g/L) and lipase activity (7531&#xa0;U/L) were obtained in YP*D medium with PC-PET and DMSO. During cultivation in this medium, TPA, MHET, and BHET were detected, demonstrating PET depolymerization along <i>Y. lipolytica</i> growth with DMSO. These results show that DMSO contributes to PET depolymerization by <i>Y. lipolytica</i>, increasing cell concentration, adhesion to PET particles, and enzyme production.</p>

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The use of dimethyl sulfoxide (DMSO) to increase PET microbial degradation by Yarrowia lipolytica IMUFRJ 50682

  • 期刊论文

  • 发表日期：

    2025年8月28日

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  • Alanna Botelho,
  • Adrian Chaves Penha,
  • Lucas Tadeu N. da Silva Rocha,
  • Ariane Gaspar Santos,
  • Danuza N. Moysés,
  • Aline Machado de Castro,
  • Maria Alice Z. Coelho,
  • Priscilla F. F. Amaral

摘要

Yarrowia lipolytica has been studied for poly (ethylene terephthalate) (PET) depolymerization, but the slow kinetics must be improved for large-scale applications. Here, dimethyl sulfoxide (DMSO) was added to a medium containing post-consumer PET (PC-PET) or the monomers terephthalic acid (TPA), bis(hydroxy-ethylene) terephthalate (BHET), and methyl-2-hydroxy ethylene terephthalate (MHET) to increase its solubility and improve depolymerization. The MIC test indicated 5% of DMSO as the maximum non-toxic concentration for Y. lipolytica cultivation. Cell viability on yeast nitrogen-based (YNB) medium was higher with MHET (94%). Cell growth in YNB medium and PC-PET was only detected with DMSO. When PC-PET was used as an additional carbon source, cell growth was 40% higher in the presence of DMSO (10.7 g/L), exhibiting increased adhesion of cells to PET (20%). Also, the highest extracellular lipase activity (370 U/L) was found with DMSO and PC-PET in flasks. In a bioreactor, higher cell growth (32.6 g/L) and lipase activity (7531 U/L) were obtained in YP*D medium with PC-PET and DMSO. During cultivation in this medium, TPA, MHET, and BHET were detected, demonstrating PET depolymerization along Y. lipolytica growth with DMSO. These results show that DMSO contributes to PET depolymerization by Y. lipolytica, increasing cell concentration, adhesion to PET particles, and enzyme production.