<p>Endozoochory is a type of mutualistic seed dispersal in which animals ingest seeds (usually contained in fruits) and then pass them in their feces, effectively distributing them to new locations. Some seed characteristics may be altered by the oral apparatus and digestive system of frugivores, resulting in physiological changes due to pulp removal or mechanical changes due to scarification of the testa, which affect seed integrity and germination. The cactus <i>Pilosocereus leucocephalus</i> produces fleshy fruits containing hundreds of seeds that are consumed by birds and mammals, which may differentially influence seed damage. Thus, we experimentally evaluated the proportion and germination of intact (whole) seeds found in the feces of representative species of these two frugivore groups. For this, at least two individuals per frugivore species (<i>Psilorhinus morio</i>, <i>Campylorhynchus rufinucha</i>, <i>Artibeus jamaicensis</i>, and <i>Peromyscus mexicanus</i>) were kept in captivity and fed with ripe <i>P. leucocephalus</i> fruits. Physical damage and germination under controlled conditions were compared between frugivore species and two control groups (non-ingested seeds with and without pulp). The proportion of intact seeds defecated, in descending order, was <i>P. morio</i>, <i>C. rufinucha,</i> and <i>A. jamaicensis</i>, while all the seeds found in the feces of <i>P. mexicanus</i> were destroyed. The germination percentages observed in the treatments, from higher to lower, were <i>P. morio</i>, control without pulp, <i>A. jamaicensis</i>, <i>C. rufinucha</i>, and control with pulp. These results suggest a gradient in seed dispersal quality, which is mutualistic in the birds and bats<i>,</i> and differentially affects seed integrity and germination, and antagonistic in rodents.</p>