Scholarlink

<p>Trypanosomatids of the <i>Schizotrypanum</i> clade display high ecological and evolutionary diversity, yet their sylvatic cycles remain understudied in many Neotropical biomes. We conducted the first integrative molecular survey of <i>Trypanosoma</i> diversity in a conserved seasonal tropical forest in the Yucatán Peninsula, Mexico. We screened <i>Triatoma dimidiata</i> vectors and small mammal hosts (rodents and bats) using satellite DNA (SatDNA) for <i>Trypanosoma cruzi</i> detection, Miniexon PCR for <i>T. cruzi</i> discrete typing unit (DTU) identification, and 18&#xa0;S rRNA sequencing to identify other <i>Trypanosoma</i> species in bats. <i>Trypanosoma cruzi</i> was detected by SatDNA in 81.8% of collected vectors (95% CI: 75.07–87.38%; <i>n</i> = 165) and in 46.6% of rodents (95% CI: 36.7–56.7%, <i>n</i> = 103) and in 46.2% of bats (95% CI: 38.56–53.97%; <i>n</i> = 171). Discrete typing unit (DTU) analysis revealed that <i>T. cruzi</i> DTU IV (TcIV) predominated in the conserved tropical forest, being mainly associated with vectors and rodents, whereas TcI was more frequent in bats. Phylogenetic reconstruction based on 18&#xa0;S rRNA revealed multiple bat-associated lineages, including sequences related to <i>T. wauwau</i>, <i>T. madeirae</i>, and Neobat clades—reported here for the first time in Mexico. Our results underscore the role of protected forests as reservoirs of trypanosomatid diversity and the relevance of multi-host surveillance to better understand transmission dynamics. This study contributes novel insights into the ecology, biogeography, and evolutionary history of <i>Trypanosoma</i> spp. in Mesoamerica and supports efforts to strengthen early detection of potential zoonotic threats.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bat-associated Trypanosoma diversity, geographic extension of known clades, and predominance of T. cruzi TcIV in a conserved tropical forest of southeastern Mexico

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月10日

查看全文
  • Martha P. Ibarra-López,
  • Román Espinal-Palomino,
  • César R. Rodríguez-Luna,
  • Joel Moo-Millán,
  • Etienne Waleckx,
  • Rodolfo Dirzo,
  • Salvador Montiel,
  • Víctor M. Vidal-Martínez,
  • Carlos N. Ibarra-Cerdeña

摘要

Trypanosomatids of the Schizotrypanum clade display high ecological and evolutionary diversity, yet their sylvatic cycles remain understudied in many Neotropical biomes. We conducted the first integrative molecular survey of Trypanosoma diversity in a conserved seasonal tropical forest in the Yucatán Peninsula, Mexico. We screened Triatoma dimidiata vectors and small mammal hosts (rodents and bats) using satellite DNA (SatDNA) for Trypanosoma cruzi detection, Miniexon PCR for T. cruzi discrete typing unit (DTU) identification, and 18 S rRNA sequencing to identify other Trypanosoma species in bats. Trypanosoma cruzi was detected by SatDNA in 81.8% of collected vectors (95% CI: 75.07–87.38%; n = 165) and in 46.6% of rodents (95% CI: 36.7–56.7%, n = 103) and in 46.2% of bats (95% CI: 38.56–53.97%; n = 171). Discrete typing unit (DTU) analysis revealed that T. cruzi DTU IV (TcIV) predominated in the conserved tropical forest, being mainly associated with vectors and rodents, whereas TcI was more frequent in bats. Phylogenetic reconstruction based on 18 S rRNA revealed multiple bat-associated lineages, including sequences related to T. wauwau, T. madeirae, and Neobat clades—reported here for the first time in Mexico. Our results underscore the role of protected forests as reservoirs of trypanosomatid diversity and the relevance of multi-host surveillance to better understand transmission dynamics. This study contributes novel insights into the ecology, biogeography, and evolutionary history of Trypanosoma spp. in Mesoamerica and supports efforts to strengthen early detection of potential zoonotic threats.

Graphical Abstract