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<p>Cystic echinococcosis (CE), caused by the larval stage of <i>Echinococcus granulosus</i> sensu lato (s.l.), remains a significant zoonotic parasitic infection worldwide. This study provides a preliminary investigation of the nuclear <i>Hsp90</i> gene diversity within the <i>E. granulosus</i> s.l. complex to evaluate its potential use for species identification. Forty-nine DNA samples of the G1 genotype from human and animal CE cysts, two G3 genotype samples, one of <i>Echinococcus ortleppi</i>, four of <i>Echinococcus canadensis</i> (G7), and four samples of other <i>Taenia</i> species (<i>Echinococcus multilocularis</i>, <i>Taenia hydatigena</i>, <i>Taenia pisiformis</i>, and <i>Taenia ovis</i>) were analyzed. Four primer pairs were designed to amplify the <i>Hsp90</i> gene, followed by PCR amplification, DNA sequencing, and phylogenetic analysis. Successful amplification and sequencing of nearly the entire <i>Hsp90</i> gene revealed a single nucleotide polymorphism (SNP) at position 222 conserved across all genotypes. Notably, significant genetic variations were observed between <i>E. ortleppi</i> (G5 genotype) and <i>E. canadensis</i> (G7 genotype) compared to <i>E. granulosus</i> sensu stricto (G1 and G3 genotypes). Phylogenetic analysis confirmed clustering consistent with established taxonomic relationships, with G1 and G3 forming a cluster, and G5 and G7 forming a distinct group. The findings suggest that the nuclear <i>Hsp90</i> gene could be used as an additional marker for species-level differentiation within the <i>E. granulosus</i> s.l. complex.</p>

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Preliminary investigation of heat shock protein 90 gene diversity in Echinococcus granulosus sensu lato: a potential nuclear marker for species identification

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月27日

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  • Zemzemi Lamia,
  • Oudni-M’rad Myriam,
  • Châabane-Banaoues Raja,
  • Kamoun Ines,
  • Gérald Umhang,
  • Abdelghani Mohamed Hedi,
  • Sayadi Taoufik,
  • Messaoud Marwa,
  • Sahnoun Lassaad,
  • Franck Boué,
  • Babba Hamouda,
  • M’rad Selim

摘要

Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus sensu lato (s.l.), remains a significant zoonotic parasitic infection worldwide. This study provides a preliminary investigation of the nuclear Hsp90 gene diversity within the E. granulosus s.l. complex to evaluate its potential use for species identification. Forty-nine DNA samples of the G1 genotype from human and animal CE cysts, two G3 genotype samples, one of Echinococcus ortleppi, four of Echinococcus canadensis (G7), and four samples of other Taenia species (Echinococcus multilocularis, Taenia hydatigena, Taenia pisiformis, and Taenia ovis) were analyzed. Four primer pairs were designed to amplify the Hsp90 gene, followed by PCR amplification, DNA sequencing, and phylogenetic analysis. Successful amplification and sequencing of nearly the entire Hsp90 gene revealed a single nucleotide polymorphism (SNP) at position 222 conserved across all genotypes. Notably, significant genetic variations were observed between E. ortleppi (G5 genotype) and E. canadensis (G7 genotype) compared to E. granulosus sensu stricto (G1 and G3 genotypes). Phylogenetic analysis confirmed clustering consistent with established taxonomic relationships, with G1 and G3 forming a cluster, and G5 and G7 forming a distinct group. The findings suggest that the nuclear Hsp90 gene could be used as an additional marker for species-level differentiation within the E. granulosus s.l. complex.