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<p>The control of <i>Aedes albopictus</i> (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the <i>Krüppel homolog 1 (Kr-h1)</i> gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized <i>Aedes albopictus Kr-h1</i>(<i>AalbKr-h1</i>), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, <i>AalbKr-h1</i> was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated <i>AalbKr-h1</i> expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the <i>AalbKr-h1</i> gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.</p> Graphical Abstract <p></p>

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Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月22日

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  • Ya-hui Chen,
  • Cheng Wu,
  • Yu-yang Xie,
  • Yan-hui Zhang,
  • Xi-tong Huang,
  • Fen Hu,
  • Li-Hua Xie

摘要

The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.

Graphical Abstract