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Background <p>Brahmi (Bacopa monnieri) plant preparation (BPP) has been recognizedfor its immunomodulatory capabilities, but its molecular and epigenetic effects onimmune cells in non-small cell lung cancer (NSCLC) remain unknown.</p> Objective <p>Thisstudy investigated the way BPP influenced the molecular phenotype and functionalprofile of monocyte-derived dendritic cells (DCs) derived from an NSCLC patient anda healthy donor. </p> Result <p>BPP treatment enhanced DC maturation, as demonstrated byelevated levels of CD1d and co-stimulatory molecules (CD80/CD86), and conditionedsupernatants that efficiently activated autologous T cells. BPP increased proinflammatorysignaling molecules STAT1, STAT2, STAT4, and total NF-κB, whiledecreasing STAT3, STAT5, STAT6, and the DNA-repair protein DNAPKcs.Transcriptional analysis indicated a Th1-oriented immune program, characterized bythe up-regulation of TBX21, IFNG, and TP53, alongside the down-regulation ofGATA3, FOXP3, RORC, IL10, and MYC. Chromatin profiling revealed an enrichmentof H3K4me3 and H3K14ac, alongside p53 recruitment at the IFNG/TBX21 loci,accompanied by diminished levels of H3K27me3, HDAC2, and reduced promoteroccupancy by c-Myc and NF-κB. BPP enhanced the secretion of IFN-γ, IL-2, and TNF-α while diminishing IL-4, IL-10, and IL-17, establishing a pro-inflammatoryenvironment indicative of Th1-type polarization. Co-culture experiments validatedincreased cytotoxic T-cell activity against A549 cells (NSCLC cells), assessed throughLDH release and statistically confirmed via ANOVA with Tukey post-hoc analysis.</p> Conclusion <p>Overall, these findings demonstrate the ability of BPP to influence dendritic and T-cell responses in NSCLC via coordinated transcriptional and chromatinremodelling activities.</p> Graphical abstract <p></p>

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Brahmi (Bacopa monnieri) plant preparation facilitates to enhance the activities of dendritic cells to control non-small cell lung cancer (NSCLC)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月22日

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  • R. Ilaya Kumar,
  • Kavya Jain,
  • Prashasti Arora,
  • Harshnna Gururajan,
  • Karan Raj Rai,
  • Oishi Mukherjee,
  • Melvin George,
  • Koustav Sarkar

摘要

Background

Brahmi (Bacopa monnieri) plant preparation (BPP) has been recognizedfor its immunomodulatory capabilities, but its molecular and epigenetic effects onimmune cells in non-small cell lung cancer (NSCLC) remain unknown.

Objective

Thisstudy investigated the way BPP influenced the molecular phenotype and functionalprofile of monocyte-derived dendritic cells (DCs) derived from an NSCLC patient anda healthy donor.

Result

BPP treatment enhanced DC maturation, as demonstrated byelevated levels of CD1d and co-stimulatory molecules (CD80/CD86), and conditionedsupernatants that efficiently activated autologous T cells. BPP increased proinflammatorysignaling molecules STAT1, STAT2, STAT4, and total NF-κB, whiledecreasing STAT3, STAT5, STAT6, and the DNA-repair protein DNAPKcs.Transcriptional analysis indicated a Th1-oriented immune program, characterized bythe up-regulation of TBX21, IFNG, and TP53, alongside the down-regulation ofGATA3, FOXP3, RORC, IL10, and MYC. Chromatin profiling revealed an enrichmentof H3K4me3 and H3K14ac, alongside p53 recruitment at the IFNG/TBX21 loci,accompanied by diminished levels of H3K27me3, HDAC2, and reduced promoteroccupancy by c-Myc and NF-κB. BPP enhanced the secretion of IFN-γ, IL-2, and TNF-α while diminishing IL-4, IL-10, and IL-17, establishing a pro-inflammatoryenvironment indicative of Th1-type polarization. Co-culture experiments validatedincreased cytotoxic T-cell activity against A549 cells (NSCLC cells), assessed throughLDH release and statistically confirmed via ANOVA with Tukey post-hoc analysis.

Conclusion

Overall, these findings demonstrate the ability of BPP to influence dendritic and T-cell responses in NSCLC via coordinated transcriptional and chromatinremodelling activities.

Graphical abstract