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Main conclusion <p>In conclusion, the present study provides preliminary functional insights into the role of OsbHLH in promoting plant growth under low-nutrient conditions. Ectopic expression of OsbHLH in Arabidopsis was associated with improved plant performance at morphological, physiological, biochemical, and molecular levels under sub-optimal nutrient regimes. Taken together, the study indicates that OsbHLH could be a key component of the regulatory framework of plant-PGPR interactions and nutrient stress responses; further targeted studies are required to conclusively establish its precise functional role and underlying mechanisms.</p> Abstract <p>Plant growth-promoting rhizobacteria (PGPR) enhance plant performance under environmentally adverse conditions. In the present study, we analyzed the functional relevance of <i>OsbHLH</i> (OsbHLH63) gene that was notably upregulated in rice following SN13 inoculation under nutrient-deficient conditions. Ectopic expression of <i>OsbHLH</i> in <i>Arabidopsis thaliana</i> resulted in improved growth, and enhanced physio-biochemical attributes under nutrient-deficient conditions. Transgenic plants also exhibited modifications in tissue organization, cellular structure, and lignification patterns. Gene expression analysis revealed differential expression of genes involved in nutrient uptake and transport (<i>IRT1, PHR1, ZNE, NRT</i>, and <i>KUP</i>), lignin biosynthesis (<i>CAD1, CCR1,</i> and <i>COMT</i>), and carbohydrate metabolism (<i>PK1, PEPC1, GDH, FBP</i>, and <i>ENO</i>). Additionally, GC–MS-based metabolomic profiling revealed 40 significantly affected metabolites associated with galactose metabolism, propanoate metabolism, amino acid biosynthesis, the pentose phosphate pathway, the TCA cycle, and glycolysis/gluconeogenesis. Collectively, these findings affirm the potential involvement of <i>OsbHLH</i> in nutrient stress adaptation and in recapitulating key aspects of SN13-induced responses observed in rice, although further targeted investigations are required to validate its regulatory function in plant–microbe interactions and stress tolerance.</p>

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Bacillus amyloliquefaciens-responsive rice basic helix–loop–helix gene acts as putative regulator of phenylpropanoid pathway, carbohydrate metabolism, and ion homeostasis under nutrient-deficient conditions

  • 期刊论文

  • 发表日期：

    2026年5月18日

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  • Nikita Bisht,
  • Harshita Joshi,
  • Anshu Anshu,
  • Shanshank Kumar Mishra,
  • Poonam C. Singh,
  • Puneet Singh Chauhan

摘要

Main conclusion

In conclusion, the present study provides preliminary functional insights into the role of OsbHLH in promoting plant growth under low-nutrient conditions. Ectopic expression of OsbHLH in Arabidopsis was associated with improved plant performance at morphological, physiological, biochemical, and molecular levels under sub-optimal nutrient regimes. Taken together, the study indicates that OsbHLH could be a key component of the regulatory framework of plant-PGPR interactions and nutrient stress responses; further targeted studies are required to conclusively establish its precise functional role and underlying mechanisms.

Abstract

Plant growth-promoting rhizobacteria (PGPR) enhance plant performance under environmentally adverse conditions. In the present study, we analyzed the functional relevance of OsbHLH (OsbHLH63) gene that was notably upregulated in rice following SN13 inoculation under nutrient-deficient conditions. Ectopic expression of OsbHLH in Arabidopsis thaliana resulted in improved growth, and enhanced physio-biochemical attributes under nutrient-deficient conditions. Transgenic plants also exhibited modifications in tissue organization, cellular structure, and lignification patterns. Gene expression analysis revealed differential expression of genes involved in nutrient uptake and transport (IRT1, PHR1, ZNE, NRT, and KUP), lignin biosynthesis (CAD1, CCR1, and COMT), and carbohydrate metabolism (PK1, PEPC1, GDH, FBP, and ENO). Additionally, GC–MS-based metabolomic profiling revealed 40 significantly affected metabolites associated with galactose metabolism, propanoate metabolism, amino acid biosynthesis, the pentose phosphate pathway, the TCA cycle, and glycolysis/gluconeogenesis. Collectively, these findings affirm the potential involvement of OsbHLH in nutrient stress adaptation and in recapitulating key aspects of SN13-induced responses observed in rice, although further targeted investigations are required to validate its regulatory function in plant–microbe interactions and stress tolerance.