Scholarlink

Main Conclusion <p>The high-quality <i>L. discolor</i> genome clarifies orchid phylogeny and provides immediate targets for breeding and biosynthetic engineering of medicinal metabolites.</p> Abstract <p><i>Ludisia discolor</i> is a valued orchid for ornamental veined foliage and documented medicinal uses, yet genomic resources are lacking. We generated the first chromosome-level assembly of 696.37&#xa0;Mb with scaffold N50 33.14&#xa0;Mb and 94.55% BUSCO completeness by integrating PacBio HiFi, Hi-C and Illumina reads; 91.86% of sequences were anchored to 22 chromosomes. Annotation yielded 20,552 protein-coding genes and 70.9% repetitive content dominated by LTR-retrotransposons. Comparative analysis revealed 157 species-specific gene families and significant expansion of terpenoid and flavonoid biosynthetic clusters, supporting its pharmacological potential. Phylogenomics placed <i>Ludisia</i> sister to <i>Platanthera</i> (Orchidoideae) with divergence ~ 39.5&#xa0;Mya. Positive selection was detected in 123 genes enriched for chromatin remodeling and nuclear transport, reflecting adaptation to shaded, nutrient-limited habitats. This reference genome provides a foundational resource for understanding orchid evolution, molecular breeding, and metabolic engineering of bioactive compounds in <i>L. discolor</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genomic characterization, annotation, and comparative analysis of Ludisia discolor reveal its evolutionary and functional traits

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月17日

查看全文
  • Qi Yang,
  • Kunxiu Cai,
  • Junjie Yang,
  • Tianxiang Zhang,
  • Luan Li,
  • Fenfen Wang,
  • Zhendong Chen,
  • Tao Zheng

摘要

Main Conclusion

The high-quality L. discolor genome clarifies orchid phylogeny and provides immediate targets for breeding and biosynthetic engineering of medicinal metabolites.

Abstract

Ludisia discolor is a valued orchid for ornamental veined foliage and documented medicinal uses, yet genomic resources are lacking. We generated the first chromosome-level assembly of 696.37 Mb with scaffold N50 33.14 Mb and 94.55% BUSCO completeness by integrating PacBio HiFi, Hi-C and Illumina reads; 91.86% of sequences were anchored to 22 chromosomes. Annotation yielded 20,552 protein-coding genes and 70.9% repetitive content dominated by LTR-retrotransposons. Comparative analysis revealed 157 species-specific gene families and significant expansion of terpenoid and flavonoid biosynthetic clusters, supporting its pharmacological potential. Phylogenomics placed Ludisia sister to Platanthera (Orchidoideae) with divergence ~ 39.5 Mya. Positive selection was detected in 123 genes enriched for chromatin remodeling and nuclear transport, reflecting adaptation to shaded, nutrient-limited habitats. This reference genome provides a foundational resource for understanding orchid evolution, molecular breeding, and metabolic engineering of bioactive compounds in L. discolor.