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Main conclusion <p>A genetic complementation test using the <i>Arabidopsis Pelota1</i> knock-out mutant revealed that <i>pepy-1</i> derived from the begomovirus-resistant <i>Capsicum</i> is a leaky pelota allele with partial loss of function.</p> Abstract <p>We previously identified <i>pepy-1</i>, a begomovirus (family <i>Geminiviridae</i>) resistance gene in <i>Capsicum</i>, as a putative loss-of-function allele of pelota. Here, we performed a genetic complementation assay using the <i>Arabidopsis Pelota1</i> knockout mutant SALK_124403, which is resistant to beet curly top virus (BCTV; family <i>Geminiviridae</i>, genus <i>Curtovirus</i>). Introduction of the susceptible allele (<i>Pepy-1</i>) restored susceptibility, whereas expression of the resistant allele (<i>pepy-1</i>) slightly compromised resistance, allowing limited viral replication but still conferring higher resistance than in Col-0. Thus, <i>pepy-1</i> functions as a leaky allele that confers partial susceptibility, thereby diminishing—but not abolishing—resistance to BCTV. The delicate balance of this leaky allele confers virus resistance with minimal impact on growth, making it well-suited for use in breeding programs.</p>

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Functional analysis of the leaky pelota allele pepy-1 from Capsicum reveals partial geminivirus resistance in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年10月27日

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  • Mika Onouchi,
  • Nadya Syafira Pohan,
  • Elly Kesumawati,
  • Sota Koeda

摘要

Main conclusion

A genetic complementation test using the Arabidopsis Pelota1 knock-out mutant revealed that pepy-1 derived from the begomovirus-resistant Capsicum is a leaky pelota allele with partial loss of function.

Abstract

We previously identified pepy-1, a begomovirus (family Geminiviridae) resistance gene in Capsicum, as a putative loss-of-function allele of pelota. Here, we performed a genetic complementation assay using the Arabidopsis Pelota1 knockout mutant SALK_124403, which is resistant to beet curly top virus (BCTV; family Geminiviridae, genus Curtovirus). Introduction of the susceptible allele (Pepy-1) restored susceptibility, whereas expression of the resistant allele (pepy-1) slightly compromised resistance, allowing limited viral replication but still conferring higher resistance than in Col-0. Thus, pepy-1 functions as a leaky allele that confers partial susceptibility, thereby diminishing—but not abolishing—resistance to BCTV. The delicate balance of this leaky allele confers virus resistance with minimal impact on growth, making it well-suited for use in breeding programs.