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Main conclusion <p>Silencing the microtubule-associated protein PlWDL2 in herbaceous peony led to a decrease in stem strength by affecting xylem development.</p> Abstract <p>Stem strength is an important factor affecting the quality of herbaceous peony (<i>Paeonia lactiflora</i> Pall.) cut flowers. To investigate the effect of microtubule-associated proteins on <i>P. lactiflora</i> stem strength, we identified <i>PlWDL2</i>, a WAVE-DAMPENED 2/WAVE-DAMPENED 2-LIKE (WVD2/WDL) family gene encoding a 340 amino acid protein with conserved KLEEK motif. Quantitative real-time PCR (qRT-PCR) revealed that <i>PlWDL2</i> expression was progressively upregulated during <i>P. lactiflora</i> stem development. In vitro co-sedimentation assays confirmed microtubule-binding capacity of PlWDL2 and its intrinsically disordered regions (IDRs) though IDRs exhibited attenuated binding correlated with shorter hydrophobic patches. Additionally, the <i>PlWDL2</i>-silenced <i>P. lactiflora</i> exhibited decreased stem strength. Further microstructure observation of the stems showed that xylem thickness, number of layers, and the proportion of xylem area and xylem cell area in the <i>PlWDL2</i>-silenced <i>P. lactiflora</i> were significantly reduced. These findings demonstrate that the microtubule-associated protein PlWDL2 enhances stem strength in <i>P. lactiflora</i> by promoting xylem development. This study lays a foundation for future studies on the mechanism of <i>P. lactiflora</i> stem development from the relationship between microtubule-associated proteins and microtubules.</p>

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Microtubule-associated protein PlWDL2 positively promotes stem strength in herbaceous peony

  • 期刊论文

  • 发表日期：

    2025年10月26日

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  • Ziao Hu,
  • Yi Qian,
  • Daqiu Zhao,
  • Jun Tao

摘要

Main conclusion

Silencing the microtubule-associated protein PlWDL2 in herbaceous peony led to a decrease in stem strength by affecting xylem development.

Abstract

Stem strength is an important factor affecting the quality of herbaceous peony (Paeonia lactiflora Pall.) cut flowers. To investigate the effect of microtubule-associated proteins on P. lactiflora stem strength, we identified PlWDL2, a WAVE-DAMPENED 2/WAVE-DAMPENED 2-LIKE (WVD2/WDL) family gene encoding a 340 amino acid protein with conserved KLEEK motif. Quantitative real-time PCR (qRT-PCR) revealed that PlWDL2 expression was progressively upregulated during P. lactiflora stem development. In vitro co-sedimentation assays confirmed microtubule-binding capacity of PlWDL2 and its intrinsically disordered regions (IDRs) though IDRs exhibited attenuated binding correlated with shorter hydrophobic patches. Additionally, the PlWDL2-silenced P. lactiflora exhibited decreased stem strength. Further microstructure observation of the stems showed that xylem thickness, number of layers, and the proportion of xylem area and xylem cell area in the PlWDL2-silenced P. lactiflora were significantly reduced. These findings demonstrate that the microtubule-associated protein PlWDL2 enhances stem strength in P. lactiflora by promoting xylem development. This study lays a foundation for future studies on the mechanism of P. lactiflora stem development from the relationship between microtubule-associated proteins and microtubules.