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Main conclusion <p><i>BroNR8</i> regulates salt tolerance in <i>Arabidopsis</i> by upregulating <i>AtWRKY54</i> and disrupting ROS homeostasis, revealing a key mechanism for salt sensitivity and offering insights for breeding salt-tolerant crops.</p> Abstract <p>Salt stress, which causes ion toxicity and oxidative damage in plants, severely threatens agricultural productivity and certain ecosystems. The regulatory network and mechanisms underlying plant responses to salt stress must be more thoroughly characterized. Many plant long non-coding RNAs (lncRNAs) transcribed by RNA polymerase II (Pol II) have been identified, with important roles in various physiological processes. The lncRNA <i>BroNR8</i> may be a 270 nt long transcript transcribed by Pol III in broccoli. It is highly expressed in the root elongation zone of germinating seeds, and its accumulation is significantly increased by salt and abscisic acid (ABA) treatments. <i>BroNR8</i> overexpression in <i>Arabidopsis</i> leads to a significant decrease in the tolerance of germinating seeds to salt stress in an ABA-independent manner. It also inhibits the maintenance of ROS homeostasis under saline conditions, thereby compromising the plant's capacity to scavenge endogenous H<sub>2</sub>O<sub>2</sub>. In response to salt stress, <i>AtWRKY54</i> expression in <i>BroNR8</i>-<i>At</i>OX increased significantly. Moreover, germinating seeds of <i>wrky54</i> were highly tolerant to salt and H<sub>2</sub>O<sub>2</sub>. Furthermore, antioxidant enzyme activities in <i>wrky54</i> increased significantly, while the ROS content decreased significantly. These results suggest that <i>BroNR8</i> may affect <i>Arabidopsis</i> tolerance to salt stress by regulating AtWRKY54-mediated ROS homeostasis. The <i>BroNR8</i>/<i>AtWRKY54</i>/ROS module detected in this study may help to clarify the molecular network regulating plant salt stress responses mediated by ncRNAs. The study findings may provide breeders with new gene resources for developing stress-resistant varieties.</p>

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Broccoli long non-coding RNA BroNR8 affects salt tolerance by mediating AtWRKY54-related reactive oxygen species homeostasis in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年9月29日

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  • Hongli Yuan,
  • Shengyi Liu,
  • Shuting Ma,
  • Jiafeng Yu,
  • Di Huang,
  • Ziguang Liu,
  • Shuang Li,
  • Yasushi Yukawa,
  • Juan Wu

摘要

Main conclusion

BroNR8 regulates salt tolerance in Arabidopsis by upregulating AtWRKY54 and disrupting ROS homeostasis, revealing a key mechanism for salt sensitivity and offering insights for breeding salt-tolerant crops.

Abstract

Salt stress, which causes ion toxicity and oxidative damage in plants, severely threatens agricultural productivity and certain ecosystems. The regulatory network and mechanisms underlying plant responses to salt stress must be more thoroughly characterized. Many plant long non-coding RNAs (lncRNAs) transcribed by RNA polymerase II (Pol II) have been identified, with important roles in various physiological processes. The lncRNA BroNR8 may be a 270 nt long transcript transcribed by Pol III in broccoli. It is highly expressed in the root elongation zone of germinating seeds, and its accumulation is significantly increased by salt and abscisic acid (ABA) treatments. BroNR8 overexpression in Arabidopsis leads to a significant decrease in the tolerance of germinating seeds to salt stress in an ABA-independent manner. It also inhibits the maintenance of ROS homeostasis under saline conditions, thereby compromising the plant's capacity to scavenge endogenous H2O2. In response to salt stress, AtWRKY54 expression in BroNR8-AtOX increased significantly. Moreover, germinating seeds of wrky54 were highly tolerant to salt and H2O2. Furthermore, antioxidant enzyme activities in wrky54 increased significantly, while the ROS content decreased significantly. These results suggest that BroNR8 may affect Arabidopsis tolerance to salt stress by regulating AtWRKY54-mediated ROS homeostasis. The BroNR8/AtWRKY54/ROS module detected in this study may help to clarify the molecular network regulating plant salt stress responses mediated by ncRNAs. The study findings may provide breeders with new gene resources for developing stress-resistant varieties.